Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.2 extracted from

  • Miller, B.G.; Raines, R.T.
    Identifying latent enzyme activities: substrate ambiguity within modern bacterial sugar kinases (2004), Biochemistry, 43, 6387-6392.
    View publication on PubMed
Search for more literature for 2.7.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of genes nanK, yajF, and ycfX in glk-deficient mutant strain BM5340(DE3) Escherichia coli K-12

Protein Variants

Protein Variants Comment Organism
additional information construction of an enzyme-deficient glk mutant strain BM5340(DE3), the mutant strain can be complemented by expression of Escherichia coli genes nanK, yajF, and ycfX, which express proteins with 4fold lower glucokinase activity and ambiguous substrate specificities, overview Escherichia coli K-12

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.076
-
 D-glucose recombinant Glk, pH 7.6, 25°C Escherichia coli K-12
0.26
-
 ATP recombinant Glk, pH 7.6, 25°C Escherichia coli K-12
0.31
-
 D-glucose recombinant YajF, pH 7.6, 25°C Escherichia coli K-12
0.51
-
 ATP recombinant YajF, pH 7.6, 25°C Escherichia coli K-12
2.9
-
 ATP recombinant NanK, pH 7.6, 25°C Escherichia coli K-12
3.4
-
 ATP recombinant YcfX, pH 7.6, 25°C Escherichia coli K-12
3.8
-
 D-glucose recombinant YcfX, pH 7.6, 25°C Escherichia coli K-12
18
-
 D-glucose recombinant NanK, pH 7.6, 25°C Escherichia coli K-12

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Escherichia coli K-12
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-glucose Escherichia coli K-12
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
 several strains
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
 Escherichia coli K-12 ADP + D-glucose 6-phosphate
-
 ?
additional information recombinant enzymes NanK and YajF also utilize N-acetyl-D-mannosamine and D-fructose as substrates, respectively Escherichia coli K-12 ?
-
 ?

Synonyms

Synonyms Comment Organism
glk
-
 Escherichia coli K-12

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli K-12

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.1
-
 D-glucose recombinant NanK, pH 7.6, 25°C Escherichia coli K-12
9.2
-
 D-glucose recombinant YcfX, pH 7.6, 25°C Escherichia coli K-12
12
-
 D-glucose recombinant Glk, pH 7.6, 25°C Escherichia coli K-12
410
-
 D-glucose recombinant Glk, pH 7.6, 25°C Escherichia coli K-12

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Escherichia coli K-12

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli K-12