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Literature summary for 2.7.1.2 extracted from

  • del Castillo, T.; Ramos, J.L.; Rodriguez-Herva, J.J.; Fuhrer, T.; Sauer, U.; Duque, E.
    Convergent peripheral pathways catalyze initial glucose catabolism in Pseudomonas putida: genomic and flux analysis (2007), J. Bacteriol., 189, 5142-5152.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.2

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is 2.12fold induced by growth on glucose compared to citrate Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
gene glk is organized in gene clusters, transcriptomic analysis of KT2440 in response to glucose, overview Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information site-specific homologous inactivation of gene glk to construct a mutant strain, activity of the enzyme in isogenic mutants with mutations in the glucose metabolism network, glk mutants exhibit a lower total carbon consumption rate than the parental strain, overview Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pseudomonas putida 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-glucose Pseudomonas putida the enzyme initiates the intracellular glucose catabolism/metabolism, overview ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose Pseudomonas putida KT 2240 the enzyme initiates the intracellular glucose catabolism/metabolism, overview ADP + D-glucose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 and isogenic mutant strains, gene glk
-
Pseudomonas putida KT 2240
-
 and isogenic mutant strains, gene glk
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity of the enzyme in isogenic mutants with mutations in the glucose metabolism network, overview Pseudomonas putida
0.0018
-
 strain KT2440, grown on citrate Pseudomonas putida
0.0211
-
 strain KT2440, grown on glucose Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
 Pseudomonas putida ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose the enzyme initiates the intracellular glucose catabolism/metabolism, overview Pseudomonas putida ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose
-
 Pseudomonas putida KT 2240 ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose the enzyme initiates the intracellular glucose catabolism/metabolism, overview Pseudomonas putida KT 2240 ADP + D-glucose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
glk
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Pseudomonas putida