Crystallization (Comment) | Organism |
---|---|
crystal structures in complexes with inhibitors 2-[[(benzyloxy)carbonyl]amino]-2-deoxy-beta-D-glucopyranose, 2-deoxy-2-[[3-(4-hydroxyphenyl)propanoyl]amino]-alpha-D-glucopyranose, 2-deoxy-2-([[(1,1-dioxido-1-benzothiophen-2-yl)methoxy]carbonyl]amino)-beta-D-glucopyranose. The analogues all share a common glucose moietythat preserves key enzyme-substrate hydrogen bonding interactions with the monosaccharide hydroxyl groups from C1, C3, C4,and C6. The C2 hydroxyl is replaced by a NH group and also par-ticipates in the key hydrogen bonding | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-deoxy-2-([[(1,1-dioxido-1-benzothiophen-2-yl)methoxy]carbonyl]amino)-beta-D-glucopyranose | - |
Trypanosoma cruzi | |
2-deoxy-2-[[3-(4-hydroxyphenyl)propanoyl]amino]-alpha-D-glucopyranose | 186fold selectivity for Trypanosoma cruzi over human enzyme | Trypanosoma cruzi | |
2-[[(benzyloxy)carbonyl]amino]-2-deoxy-beta-D-glucopyranose | 245fold selectivity for Trypanosoma cruzi over human enzyme | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.95 | - |
D-glucose | His-tagged recombinant protein, pH 7.2, 22°C | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q4E4E1 | - |
- |
Trypanosoma cruzi CL Brener | Q4E4E1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Trypanosoma cruzi | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | - |
Trypanosoma cruzi CL Brener | ADP + D-glucose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucokinase 1 | - |
Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.8 | - |
D-glucose | His-tagged recombinant protein, pH 7.2, 22°C | Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00071 | - |
2-[[(benzyloxy)carbonyl]amino]-2-deoxy-beta-D-glucopyranose | His-tagged recombinant protein, pH 7.2, 22°C | Trypanosoma cruzi | |
0.0013 | - |
2-deoxy-2-[[3-(4-hydroxyphenyl)propanoyl]amino]-alpha-D-glucopyranose | His-tagged recombinant protein, pH 7.2, 22°C | Trypanosoma cruzi | |
0.0041 | - |
2-deoxy-2-([[(1,1-dioxido-1-benzothiophen-2-yl)methoxy]carbonyl]amino)-beta-D-glucopyranose | His-tagged recombinant protein, pH 7.2, 22°C | Trypanosoma cruzi |