Any feedback?
Literature summary for 2.7.1.20 extracted from
- Isolation and characterization of adenosine kinase from Leishmania donovani (1987), J. Biol. Chem., 262, 5515-5521.
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin stabilizes | Leishmania donovani |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-Methylmercaptopurine riboside | strong inhibition, substrate inhibition | Leishmania donovani |  |
| adenosine | no inhibition | Leishmania donovani | |
| ATP | free form, above 0.5 mM | Leishmania donovani | |
| deoxyadenosine | - |
Leishmania donovani | |
| deoxyguanosine | no inhibition | Leishmania donovani | |
| EDTA | - |
Leishmania donovani | |
| guanosine | no inhibition | Leishmania donovani | |
| Mg2+ | not | Leishmania donovani | |
| additional information | no inhibition by S-adenosylhomocysteine, 2'-deoxycytidine, thymidine and cytidine | Leishmania donovani | |
| NEM | moderate inhibition | Leishmania donovani | |
| Nucleosides | weak inhibition | Leishmania donovani | |
| p-hydroxymercuribenzoate | DTT reverses; strong inhibition | Leishmania donovani | |
| tubercidin | strong inhibition, substrate inhibition | Leishmania donovani | |
| uridine | no inhibition | Leishmania donovani | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00384 | - |
6-Methylmercaptopurine riboside | pH 7.5, 30ºC | Leishmania donovani | |
| 0.033 | - |
ATP | pH 7.5, 30ºC, with adenosine and ATP saturating concentrations | Leishmania donovani | |
| 0.05 | - |
ATP | - |
Leishmania donovani | |
| 0.0833 | - |
tubercidin | pH 7.5, 30ºC | Leishmania donovani | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | no activation | Leishmania donovani | |
| Ca2+ | not | Leishmania donovani | |
| Mg2+ | requirement | Leishmania donovani | |
| Mg2+ | reactive species: MgATP2- | Leishmania donovani | |
| Mn2+ | activation | Leishmania donovani | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37700 | - |
gel filtration | Leishmania donovani |
| 38250 | - |
1 * 38250, SDS-PAGE | Leishmania donovani |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | - |
UR6 strain | - |
| Leishmania donovani UR6 | - |
UR6 strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion exchange, gel filtration and affinity chromatography, 3250fold purification | Leishmania donovani |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| promastigote | - |
Leishmania donovani | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11 | - |
- |
Leishmania donovani |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 2 months | Leishmania donovani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 6-methylmercaptopurine riboside | - |
Leishmania donovani | ADP + 6-methylmercaptopurine-D-riboside 5'-phosphate | - |
? | |
| ATP + adenosine | best substrates | Leishmania donovani | ADP + AMP | - |
? | |
| ATP + adenosine | best substrates | Leishmania donovani UR6 | ADP + AMP | - |
? | |
| ATP + tubercidin | - |
Leishmania donovani | ADP + tubercidin 5'-phosphate | - |
? | |
| ATP + tubercidin | - |
Leishmania donovani UR6 | ADP + tubercidin 5'-phosphate | - |
? | |
| CTP + adenosine | CTP is a poor substrate | Leishmania donovani | CDP + AMP | - |
? | |
| CTP + adenosine | CTP is a poor substrate | Leishmania donovani UR6 | CDP + AMP | - |
? | |
| GTP + adenosine | - |
Leishmania donovani | GDP + AMP | - |
? | |
| GTP + adenosine | - |
Leishmania donovani UR6 | GDP + AMP | - |
? | |
| UTP + adenosine | UTP is a poor substrate | Leishmania donovani | UDP + AMP | - |
? | |
| UTP + adenosine | UTP is a poor substrate | Leishmania donovani UR6 | UDP + AMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 38250, SDS-PAGE | Leishmania donovani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Leishmania donovani |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the pH-optimum is a function of the ATP/Mg2+ ratio | Leishmania donovani |
| 7.5 | 8.5 | broad, dependent upon ATP/Mg2+ ratio | Leishmania donovani |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
above, inhibition, with a high ATP/Mg2+ ratio | Leishmania donovani |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Leishmania donovani | isoelectric focusing | - |
8.8 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
