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Literature summary for 2.7.1.21 extracted from
- Human deoxythymidine kinase. I. Purification and general properties of the cytoplasmic and mitochondrial isozymes derived from blast cells of acute myelocytic leukemia (1976), J. Biol. Chem., 251, 2600-2604.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition: AMP, CMP, GMP, putrescine, spermine, spermidine | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partially substitute for Mg2+ | Homo sapiens |  |
| Cu2+ | can partially substitute for Mg2+ | Homo sapiens | |
| Fe2+ | can partially substitute for Mg2+ | Homo sapiens | |
| Mg2+ | - |
Homo sapiens | |
| Mn2+ | can partially substitute for Mg2+ | Homo sapiens | |
| Zn2+ | can partially substitute for Mg2+ | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | 74000 | gel filtration | Homo sapiens |
| 84000 | 98000 | gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| rLyTK1Val-106 and rLyTK1Met-106 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blast cell | 2 forms | Homo sapiens | - |
| fibroblast | embryonal | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.124 | 0.183 | - |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + thymidine | - |
Homo sapiens | ADP + dTMP | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 40 | 40 min stable | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 7.8 | mitochondrial enzyme | Homo sapiens |
| 7.6 | 7.8 | cytoplasmic enzyme | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Homo sapiens |
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