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Literature summary for 2.7.1.21 extracted from

  • Wang, L.; Saada, A.; Eriksson, S.
    Kinetic properties of mutant human thymidine kinase 2 suggest a mechanism for mitochondrial DNA depletion myopathy (2003), J. Biol. Chem., 278, 6963-6968.
    View publication on PubMed
Search for more literature for 2.7.1.21

Cloned(Commentary)

Cloned (Comment) Organism
TK2 Homo sapiens

Protein Variants

Protein Variants Comment Organism
H121N similar subunit structure to wild-type enzyme, decreased enzyme activity Homo sapiens
I212N similar subunit structure to wild-type enzyme, less than 1% activity compared to wild-type Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
dCTP
-
 Homo sapiens
deoxcytidine
-
 Homo sapiens
dTTP
-
 Homo sapiens
thymidine
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0057
-
 thymidine H121N mutant, pH 7.6 Homo sapiens
0.011
-
 deoxycytidine wild-type and H121N mutant, pH 7.6 Homo sapiens
0.013
-
 thymidine wild-type, pH 7.6 Homo sapiens
0.018
-
 thymidine I212 mutant, pH 7.6 Homo sapiens
0.025
-
 ATP wild-type, thymidine as substrate, pH 7.6 Homo sapiens
0.037
-
 ATP wild-type, deoxycytidine as substrate, pH 7.6 Homo sapiens
0.059
-
 ATP I212 mutant, deoxycytidine as substrate, pH 7.6 Homo sapiens
0.08
-
 ATP H121N mutant, thymidine as substrate, pH 7.6 Homo sapiens
0.095
-
 ATP I212 mutant, thymidine as substrate, pH 7.6 Homo sapiens
0.162
-
 ATP H121N mutant, deoxycytidine as substrate, pH 7.6 Homo sapiens
0.568
-
 deoxycytidine I212 mutant, pH 7.6 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens O00142
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00036
-
 dCTP H121N mutant, pH 7.6, competitive with thymidine as substrate Homo sapiens
0.00079
-
 dCTP wild-type enzyme, pH 7.6, competitive with thymidine as substrate Homo sapiens
0.00087
-
 dCTP wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate Homo sapiens
0.00089
-
 dTTP H121N mutant, pH 7.6, competitive with thymidine as substrate Homo sapiens
0.00089
-
 dCTP H121N mutant, pH 7.6, competitive with deoxycytidine as substrate Homo sapiens
0.002
-
 dTTP wild-type enzyme, pH 7.6, competitive with thymidine as substrate Homo sapiens
0.0025
-
 dTTP wild-type and H121N mutant, pH 7.6, non-competitive with deoxycytidine as substrate Homo sapiens
0.0036
-
 thymidine H121N mutant, pH 7.6, competitive with deoxycytidine as substrate Homo sapiens
0.004
-
 deoxycytidine H121N mutant, pH 7.6, non-competitive with thymidine as substrate Homo sapiens
0.0049
-
 thymidine wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate Homo sapiens
0.04
-
 deoxycytidine wild-type enzyme, pH 7.6, non-competitive with thymidine as substrate Homo sapiens