Literature summary for 2.7.1.21 extracted from

O'Day, D.H.; Chatterjee-Chakraborty, M.; Wagler, S.; Myre, M.A.
Isolation and characterization of Dictyostelium thymidine kinase 1 as a calmodulin-binding protein (2005), Biochem. Biophys. Res. Commun., 331, 1494-1502.

Search for more literature for 2.7.1.21

Activating Compound

Activating Compound	Comment	Organism	Structure
Calmodulin	stimulates the enzyme by 13.8%, might modify the activity, enzyme contains a calmodulin binding domain, Ca2+ is required for binding, compounds W7 and W13 prevent stimulation, overview	Dictyostelium discoideum

Cloned(Commentary)

Cloned (Comment)	Organism
gene thyB, DNA and amino acid sequence determination and analysis, phage display, location on chromosome 5, 3 exons, 2 introns, expression in Escherichia coli strain BL21	Dictyostelium discoideum

Inhibitors

Inhibitors	Comment	Organism	Structure
W7	slight inhibitory in presence of calmodulin	Dictyostelium discoideum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Dictyostelium discoideum	5829	-
additional information	enzyme possesses no nuclear or transmembrane sequences	Dictyostelium discoideum	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	calmodulin binding binding is dependent on Ca2+	Dictyostelium discoideum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25494	-	x * 25494, recombinant enzyme, amino acid sequence calculation	Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + thymidine	Dictyostelium discoideum	-	ADP + thymidine 5'-phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Dictyostelium discoideum	Q27564	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme partially utilizing calmodulin-agarose-beads	Dictyostelium discoideum

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	enzyme is expressed during growth, but decreases in amount after starvation	Dictyostelium discoideum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + thymidine	-	Dictyostelium discoideum	ADP + thymidine 5'-phosphate	-	ir

Subunits

Subunits	Comment	Organism
?	x * 25494, recombinant enzyme, amino acid sequence calculation	Dictyostelium discoideum
More	enzyme contains a calmodulin binding domain	Dictyostelium discoideum

Synonyms

Synonyms	Comment	Organism
calmodulin-binding protein	-	Dictyostelium discoideum
CaM-binding protein	-	Dictyostelium discoideum
CaMBP	-	Dictyostelium discoideum
ThyB	-	Dictyostelium discoideum
thymidine kinase 1	-	Dictyostelium discoideum
TK1	-	Dictyostelium discoideum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Dictyostelium discoideum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Dictyostelium discoideum

Cofactor

Cofactor	Comment	Organism	Structure
ATP	P-loop binding site	Dictyostelium discoideum

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Release 2023.1 (January 2023)