Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as poly-histidine fusion protein in the pET-16b vector | Vaccinia virus |
expression in Escherichia coli as poly-histidine fusion protein in the pET-16b vector | Felid alphaherpesvirus 1 |
expression in Escherichia coli as poly-histidine fusion protein in the pET-16b vector | Canid alphaherpesvirus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
3'-azido-3'-deoxythymidine | 37°C, pH 7.6 | Felid alphaherpesvirus 1 | |
0.016 | - |
2'-deoxythymidine | 37°C, pH 7.6 | Canid alphaherpesvirus 1 | |
0.041 | - |
2'-deoxythymidine | 37°C, pH 7.6 | Felid alphaherpesvirus 1 | |
0.05 | - |
3'-azido-3'-deoxythymidine | 37°C, pH 7.6 | Canid alphaherpesvirus 1 | |
0.33 | - |
2'-deoxythymidine | 37°C, pH 7.6 | Vaccinia virus | |
0.6 | - |
3'-azido-3'-deoxythymidine | 37°C, pH 7.6 | Vaccinia virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canid alphaherpesvirus 1 | - |
- |
- |
Felid alphaherpesvirus 1 | - |
- |
- |
Vaccinia virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vaccinia virus |
- |
Felid alphaherpesvirus 1 |
- |
Canid alphaherpesvirus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine | about 115% of the activity with 2'-deoxythymidine | Canid alphaherpesvirus 1 | ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 1-beta-D-arabinofuranosylthymine | as active as with 2'-deoxythymidine | Canid alphaherpesvirus 1 | ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | - |
Vaccinia virus | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | - |
Felid alphaherpesvirus 1 | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | - |
Canid alphaherpesvirus 1 | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 3'-azido-2',3'-dideoxythymidine | about 165% of the activity with 2'-deoxythymidine | Vaccinia virus | ADP + 3'-azido-2',3'-dideoxythymidine 5'-phosphate | - |
? | |
ATP + 3'-azido-2',3'-dideoxythymidine | about 5% of the activity with 2'-deoxythymidine | Canid alphaherpesvirus 1 | ADP + 3'-azido-2',3'-dideoxythymidine 5'-phosphate | - |
? | |
ATP + 3'-azido-3'-deoxythymidine | - |
Felid alphaherpesvirus 1 | ADP + 3'-azido-3'-deoxythymidine 5'-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CHV-TK | - |
Canid alphaherpesvirus 1 |
FHV-TK | - |
Felid alphaherpesvirus 1 |
VVTK | - |
Vaccinia virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
2'-deoxythymidine | 37°C, pH 7.6 | Canid alphaherpesvirus 1 | |
0.53 | - |
3'-azido-3'-deoxythymidine | 37°C, pH 7.6 | Canid alphaherpesvirus 1 | |
0.97 | - |
3'-azido-3'-deoxythymidine | 37°C, pH 7.6 | Felid alphaherpesvirus 1 | |
6.47 | - |
2'-deoxythymidine | 37°C, pH 7.6 | Canid alphaherpesvirus 1 | |
23 | - |
2'-deoxythymidine | 37°C, pH 7.6 | Felid alphaherpesvirus 1 | |
220 | - |
2'-deoxythymidine | 37°C, pH 7.6 | Vaccinia virus | |
412 | - |
3'-azido-3'-deoxythymidine | 37°C, pH 7.6 | Vaccinia virus |