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Literature summary for 2.7.1.21 extracted from
- Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana (2012), FEBS J., 279, 3889-3897.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli KY895 cells | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00038 | - |
3'-azido-3'-deoxythymidine | isoform TK1b, pH and temperature not specified in the publication | Arabidopsis thaliana |  |
| 0.00062 | - |
thymidine | isoform TK1a, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 0.0014 | - |
thymidine | isoform TK1b, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 0.0027 | - |
3'-azido-3'-deoxythymidine | isoform TK1a, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 0.0066 | - |
deoxyuridine | isoform TK1b, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 0.015 | - |
deoxyuridine | isoform TK1a, pH and temperature not specified in the publication | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26100 | - |
x * 26100, isoform TK1a, calculated from amino acid sequence | Arabidopsis thaliana |
| 30700 | - |
x * 30700, isoform TK1b, calculated from amino acid sequence | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q8LDP6 | ecotype Columbia-0 | - |
| Arabidopsis thaliana | Q9FN47 | ecotype Columbia-0 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bud | - |
Arabidopsis thaliana | - |
| silique | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3'-azido-3'-deoxythymidine | - |
Arabidopsis thaliana | ADP + 3'-azido-3'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + deoxyuridine | - |
Arabidopsis thaliana | ADP + dUMP | - |
? | |
| ATP + thymidine | best substrate | Arabidopsis thaliana | ADP + dTMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26100, isoform TK1a, calculated from amino acid sequence | Arabidopsis thaliana |
| ? | x * 30700, isoform TK1b, calculated from amino acid sequence | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thymidine kinase 1 | - |
Arabidopsis thaliana |
| TK1 | - |
Arabidopsis thaliana |
| TK1a | isoform | Arabidopsis thaliana |
| TK1b | isoform | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
3'-azido-3'-deoxythymidine | isoform TK1b, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 0.02 | - |
thymidine | isoform TK1b, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 0.045 | - |
deoxyuridine | isoform TK1b, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 0.13 | - |
3'-azido-3'-deoxythymidine | isoform TK1a, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 0.48 | - |
thymidine | isoform TK1a, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 1.5 | - |
deoxyuridine | isoform TK1a, pH and temperature not specified in the publication | Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | TK1a/TK1b double mutants die at an early stage | Arabidopsis thaliana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.8 | - |
deoxyuridine | isoform TK1b, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 14 | - |
thymidine | isoform TK1b, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 22 | - |
3'-azido-3'-deoxythymidine | isoform TK1b, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 48 | - |
3'-azido-3'-deoxythymidine | isoform TK1a, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 150 | - |
deoxyuridine | isoform TK1a, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 770 | - |
thymidine | isoform TK1a, pH and temperature not specified in the publication | Arabidopsis thaliana | |
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