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Literature summary for 2.7.1.23 extracted from

  • Sakuraba, H.; Kawakami, R.; Ohshima, T.
    First archaeal inorganic polyphosphate/ATP-dependent NAD kinase, from hyperthermophilic archaeon Pyrococcus horikoshii: cloning, expression, and characterization (2005), Appl. Environ. Microbiol., 71, 4352-4358.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.23

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 NAD+ cosubstrate polyP27, pH 6.8, 70°C Pyrococcus horikoshii
0.4
-
 NAD+ cosubstrate ATP, pH 6.8, 70°C Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 5 mM, substrate polyP27, 57% activity, substrate ATP, 65% activity Pyrococcus horikoshii
CuCl2 5 mM, substrate polyP27, 100% activity, substrate ATP, 62% activity Pyrococcus horikoshii
MgCl2 5 mM, substrate polyP27, 100% activity, substrate ATP, 100% activity Pyrococcus horikoshii
MnCl2 5 mM, substrate polyP27, 100% activity, substrate ATP, 62% activity Pyrococcus horikoshii
NiCl2 5 mM, substrate polyP27, 80% activity, substrate ATP, 36% activity Pyrococcus horikoshii
ZnCl2 5 mM, substrate polyP27, 56% activity, substrate ATP, 78% activity Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 4 * 37000, SDS-PAGE Pyrococcus horikoshii
145000
-
 gel filtration Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58801
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pyrococcus horikoshii

Storage Stability

Storage Stability Organism
precipitation during dialysis against phosphate buffer without NaCl and imidazole, storage in 0.5 M NaCl and 0.5 M imidazole at 4°C for up to 2 months without loss of activity Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+ 75% of the activity with polyP27 Pyrococcus horikoshii ADP + NADP+
-
 ?
ATP + NADH
-
 Pyrococcus horikoshii ADP + NADPH
-
 ?
CTP + NAD+ 76% of the activity with polyP27 Pyrococcus horikoshii CDP + NADP+
-
 ?
GTP + NAD+ 111% of the activity with polyP27 Pyrococcus horikoshii GDP + NADP+
-
 ?
ITP + NAD+ 89% of the activity with polyP27 Pyrococcus horikoshii IDP + NADP+
-
 ?
polyphosphate + NAD+ activity with polyphosphates in decreasing order: polyP27, polyP32, polyP18, polyP46, polyP62, polyP5, no activity with orthophosphate or diphosphate Pyrococcus horikoshii (phosphate)n-1 + NADP+
-
 ?
TTP + NAD+ 13% of the activity with polyP27 Pyrococcus horikoshii TDP + NADP+
-
 ?
UTP + NAD+ 94% of the activity with polyP27 Pyrococcus horikoshii UDP + NADP+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 37000, SDS-PAGE Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 60 min, stable in pH-range 4.0-10.5 Pyrococcus horikoshii
95
-
 10 min, stable Pyrococcus horikoshii
100
-
 10 min, 20% residual activity Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Pyrococcus horikoshii