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Literature summary for 2.7.1.25 extracted from

  • Lee, S.; Leustek, T.
    APS kinase from Arabidopsis thaliana: genomic organization, expression, and kinetic analysis of the recombinant enzyme (1998), Biochem. Biophys. Res. Commun., 247, 171-175.
    View publication on PubMed
Search for more literature for 2.7.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as fusion protein with glutathione-S-transferase or maltose binding protein Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-phosphosulfate substrate inhibition above 0.01 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
 adenosine 5'-phosphosulfate 25°C Arabidopsis thaliana
1.9
-
 ATP 25°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast localization is suggested by an in vitro chloroplast import assay Arabidopsis thaliana 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29786
-
 x * 29786, deduced from nucleotide sequence Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q43295
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf APS kinase mRNA Arabidopsis thaliana
-
root APS kinase mRNA Arabidopsis thaliana
-

Storage Stability

Storage Stability Organism
-70°C, recombinant APS kinase fusion protein, 1 year, no loss of activity Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Arabidopsis thaliana ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?

Subunits

Subunits Comment Organism
? x * 29786, deduced from nucleotide sequence Arabidopsis thaliana