Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
162000 | - |
gel filtration | Homo sapiens |
716000 | - |
2 * 716000, MALDI-TOF mass spectrometry, native mass by gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5'-phosphosulfate | Homo sapiens | synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5'-phosphosulfate | - |
Homo sapiens | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? | |
ATP + adenosine 5'-phosphosulfate | synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate | Homo sapiens | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 716000, MALDI-TOF mass spectrometry, native mass by gel filtration | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
More | bifunctional enzyme with ATP sulfurylase activity and adenosine phosphosulfate kinase activity, catalyzes the formation of 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate | Homo sapiens |
PAPS synthetase | two isoforms, type 1 and 2 | Homo sapiens |