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Literature summary for 2.7.1.25 extracted from

  • Singh, B.; Schwartz, N.B.
    Identification and functional characterization of the novel BM-motif in the murine phosphoadenosine phosphosulfate (PAPS) synthetase (2003), J. Biol. Chem., 278, 71-75.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) Mus musculus

Protein Variants

Protein Variants Comment Organism
D87A almost complete loss of activity Mus musculus
D87E 80% loss of activity Mus musculus
D87R almost complete loss of activity Mus musculus
D89A almost complete loss of activity Mus musculus
D89E 75% loss of activity Mus musculus
D89R almost complete loss of activity Mus musculus
DN89ND switch mutant, almost complete loss of activity Mus musculus
G88A less than 30% loss of kinase activity Mus musculus
G88D almost complete loss of activity Mus musculus
G88R almost complete loss of activity Mus musculus
K97A no effect on activity Mus musculus
LD86DL switch mutant, almost complete loss of activity Mus musculus
N90A 30% loss of kinase activity Mus musculus
N90Q 30% loss of kinase activity Mus musculus
R92A complete loss of kinase activity Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + adenosine 5'-phosphosulfate Mus musculus synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate, enzyme defect causes brachymorphism, a severe growth disorder affecting skeletal elements as well as other physiological processes ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000298
-
wild type activity, specific activities given for all mutant enzymes Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5'-phosphosulfate
-
Mus musculus ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?
ATP + adenosine 5'-phosphosulfate synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate, enzyme defect causes brachymorphism, a severe growth disorder affecting skeletal elements as well as other physiological processes Mus musculus ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?

Synonyms

Synonyms Comment Organism
More bifunctional enzyme with ATP sulfurylase activity and adenosine phosphosulfate kinase activity, catalyzes the formation of 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate Mus musculus