Cloned (Comment) | Organism |
---|---|
the APS-kinase domain (25-227 amino acid resiudes) of the PAPSS1 coding region are expressed as a GST-fusion protein in Escherichia coli BL-21 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystal structures of the PAPSS1 APS-kinase domain in complex with APS and in complex with the products PAPS and ADP are solved, both structures of isolated domain, obtained in different crystal forms, reveal symmetrical dimers | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
EDTA | assay with 0.25 mM EDTA | Homo sapiens | |
K+ | assay with 100 mM KCl | Homo sapiens | |
Mg2+ | assay with 2.5 mM MgCl2 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protein solution is loaded on a Glutathione-Sepharose Fast Flow column and further purified by gel-filtration chromatography using Superdex 200 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
MgATP2- + adenylyl sulfate | - |
Homo sapiens | MgADP- + 3'-phosphoadenylyl sulfate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
3'-phosphoadenosine 5'-phosphosulfate synthetase1 | - |
Homo sapiens |
PAPSS1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |