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Literature summary for 2.7.1.25 extracted from

  • Ravilious, G.E.; Jez, J.M.
    Nucleotide binding site communication in Arabidopsis thaliana adenosine 5’-phosphosulfate kinase (2012), J. Biol. Chem., 287, 30385-30394.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with adenosine 5'-phosphosulfate, hanging drop vapor diffusion method, using 100 mM HEPES, pH 7.25, 200 mM MgCl2, and 15–17.5% (w/v) PEG 2000 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
D136A the mutant displays drastically reduced affinity for adenosine 5'-phosphosulfate compared to the wild type enzyme Arabidopsis thaliana
D136N the mutant displays drastically reduced affinity for adenosine 5'-phosphosulfate compared to the wild type enzyme Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-phosphosulfate
-
 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00048
-
 adenosine 5'-phosphosulfate wild type enzyme, at pH 7.5 and 17°C Arabidopsis thaliana
0.0007
-
 adenosine 5'-phosphosulfate mutant enzyme D136N, at pH 7.5 and 17°C Arabidopsis thaliana
0.00092
-
 adenosine 5'-phosphosulfate mutant enzyme D136A, at pH 7.5 and 17°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q43295
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography and Superdex-200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5'-phosphosulfate
-
 Arabidopsis thaliana ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Synonyms

Synonyms Comment Organism
adenosine 5Â’-phosphosulfate kinase
-
 Arabidopsis thaliana
adenylyl-sulfate kinase
-
 Arabidopsis thaliana
APS kinase
-
 Arabidopsis thaliana
APSK
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29
-
 adenosine 5'-phosphosulfate mutant enzyme D136A, at pH 7.5 and 17°C Arabidopsis thaliana
45
-
 adenosine 5'-phosphosulfate mutant enzyme D136N, at pH 7.5 and 17°C Arabidopsis thaliana
272
-
 adenosine 5'-phosphosulfate wild type enzyme, at pH 7.5 and 17°C Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0328
-
 adenosine 5'-phosphosulfate mutant enzyme D136N, at pH 7.5 and 17°C Arabidopsis thaliana
0.0375
-
 adenosine 5'-phosphosulfate wild type enzyme, at pH 7.5 and 17°C Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
31000
-
 adenosine 5'-phosphosulfate mutant enzyme D136A, at pH 7.5 and 17°C Arabidopsis thaliana
64000
-
 adenosine 5'-phosphosulfate mutant enzyme D136N, at pH 7.5 and 17°C Arabidopsis thaliana
567000
-
 adenosine 5'-phosphosulfate wild type enzyme, at pH 7.5 and 17°C Arabidopsis thaliana