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Literature summary for 2.7.1.29 extracted from
- A mechanism of covalent substrate binding in the x-ray structure of subunit K of the Escherichia coli dihydroxyacetone kinase (2003), Proc. Natl. Acad. Sci. USA, 100, 8188-8192.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| DhaK and DhaK-dihydroxacetone complex are crystallized from 80 mM sodium acetate, pH 5.0, 160 mM ammonium sulfate, 17% polyethylene glycol 4000, 15% 2-methyl-2,4-pentanediol using hanging drop vapor diffusion, crystals diffract to 1.75 A resolution | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P37349 | - |
- |
| Escherichia coli | P76014 | - |
- |
| Escherichia coli | P76015 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, ResourceQ, Superdex 200 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospho-DhaM + 3,4-dihydroxy-2-butanone | - |
Escherichia coli | dephospho-DhaM + 3-hydroxy-2-butanone-4-phosphate | - |
? |  |
| phospho-DhaM + erythrose | - |
Escherichia coli | dephospho-DhaM + erythrose 4-phosphate | - |
? | |
| phospho-DhaM + glyceraldehyde | - |
Escherichia coli | dephospho-DhaM + glyceraldehyde 2-phosphate | - |
? | |
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Release 2023.1 (January 2023)
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