General Stability | Organism |
---|---|
(NH4)2SO4 stabilizes | Haloarcula vallismortis |
stabilizing effect of KCl increases with increasing KCl concentration between 0.1 and 2.5 M (ranging from 30 to 98% of the total) | Haloarcula vallismortis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | - |
Haloarcula vallismortis | |
ADP | competitive | Haloarcula vallismortis | |
DTNB | - |
Haloarcula vallismortis | |
LiCl | - |
Haloarcula vallismortis | |
NH4Cl | - |
Haloarcula vallismortis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
ATP | pH 9.0, 37°C | Haloarcula vallismortis | |
5 | - |
D-fructose | pH 9.0, 37°C | Haloarcula vallismortis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CsCl | the enzyme is maximally active in the presence of 1.2 M KCl. Activity in the presence of RbCl and CsCl is similar to that observed in the presence of KCl | Haloarcula vallismortis | |
KCl | the enzyme is maximally active in the presence of 1.2 M KCl. Activity in the presence of RbCl and CsCl is similar to that observed in the presence of KCl | Haloarcula vallismortis | |
Mg2+ | most active at a Mg2+ concentration of 1 mM. Mg2+ can not be replaced by Mn2+. ATP/Mg2+ in the ratio of 1:2 forms the actual substrate for ketohexokinase activity | Haloarcula vallismortis | |
NaCl | stimulates, yields 50% of the activity observed in equimolar KCl | Haloarcula vallismortis | |
RbCl | the enzyme is maximally active in the presence of 1.2 M KCl. Activity in the presence of RbCl and CsCl is similar to that observed in the presence of KCl | Haloarcula vallismortis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95500 | - |
gel filtration | Haloarcula vallismortis |
100000 | - |
sucrose density gradient sedimentation analysis | Haloarcula vallismortis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | Haloarcula vallismortis | - |
ADP + D-fructose 1-phosphate | - |
? | |
ATP + D-fructose | Haloarcula vallismortis ATCC 34679 | - |
ADP + D-fructose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula vallismortis | - |
- |
- |
Haloarcula vallismortis ATCC 34679 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloarcula vallismortis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | - |
Haloarcula vallismortis | ADP + D-fructose 1-phosphate | - |
? | |
ATP + D-fructose | except for ATP, other nucleotides such as UTP, CTP, GTP, or ITP can not act as phosphoryl donors | Haloarcula vallismortis | ADP + D-fructose 1-phosphate | - |
? | |
ATP + D-fructose | - |
Haloarcula vallismortis ATCC 34679 | ADP + D-fructose 1-phosphate | - |
? | |
ATP + D-fructose | except for ATP, other nucleotides such as UTP, CTP, GTP, or ITP can not act as phosphoryl donors | Haloarcula vallismortis ATCC 34679 | ADP + D-fructose 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP:D-fructose 1-phosphotransferase | - |
Haloarcula vallismortis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Haloarcula vallismortis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
25°C, 2 h, totally inactivated in 0.1 M KCl/50 mM Tris-HCl (pH 9.0). Stabilizing effect of KCl increases with increasing KCl concentration between 0.1 and 2.5 M (ranging from 30 to 98% of the total). Addition of 50 mM phosphate aids in stabilization of ketohexokinase activity in 0.1 M KCI for about 24 h. Although it is inhibitory for expression of ketohexokinase activity, (NH4)2SO4 stabilizes | Haloarcula vallismortis |
75 | - |
30 min, 5% loss of activity | Haloarcula vallismortis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Haloarcula vallismortis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.5 | activity increases about twofold from pH 7.5 to pH 10.5 | Haloarcula vallismortis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
ADP | pH 9.0, 37°C | Haloarcula vallismortis | |
0.2 | - |
ADP | pH 7.5, 37°C | Haloarcula vallismortis |