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Literature summary for 2.7.1.30 extracted from

  • Balogun, E.O.; Inaoka, D.K.; Shiba, T.; Kido, Y.; Nara, T.; Aoki, T.; Honma, T.; Tanaka, A.; Inoue, M.; Matsuoka, S.; Michels, P.A.; Harada, S.; Kita, K.
    Biochemical characterization of highly active Trypanosoma brucei gambiense glycerol kinase, a promising drug target (2013), J. Biochem., 154, 77-84.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Trypanosoma brucei gambiense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
ADP at pH 6.8 and 37°C Trypanosoma brucei gambiense
5.54
-
sn-glycerol 3-phosphate at pH 6.8 and 37°C Trypanosoma brucei gambiense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60400
-
-
Trypanosoma brucei gambiense
119000
-
gel filtration Trypanosoma brucei gambiense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + sn-glycerol 3-phosphate Trypanosoma brucei gambiense
-
ATP + glycerol
-
r
ATP + glycerol Trypanosoma brucei gambiense
-
ADP + sn-glycerol 3-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei gambiense
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity resin column chromatography and gel filtration Trypanosoma brucei gambiense

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
lysate, at pH 6.8 and 37°C Trypanosoma brucei gambiense
32
-
after 76fold purification, at pH 6.8 and 37°C Trypanosoma brucei gambiense

Storage Stability

Storage Stability Organism
4°C, in 0.1 M sodium phosphate buffer pH 6.8 containing 10 mM MgSO4, 48 h, more than 50% loss of activity Trypanosoma brucei gambiense
4°C, in 0.1 M sodium phosphate buffer pH 6.8 containing 10 mM MgSO4, 5 mM ADP, 10 mM MgSO4 and 0.1% (v/v) glycerol, 4 weeks, no loss of activity Trypanosoma brucei gambiense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + sn-glycerol 3-phosphate
-
Trypanosoma brucei gambiense ATP + glycerol
-
r
ATP + glycerol
-
Trypanosoma brucei gambiense ADP + sn-glycerol 3-phosphate
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 60400, calculated from amino acid sequence Trypanosoma brucei gambiense

Synonyms

Synonyms Comment Organism
ATP:glycerol 3-phosphotransferase
-
Trypanosoma brucei gambiense
GLPK
-
Trypanosoma brucei gambiense

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 70
-
Trypanosoma brucei gambiense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Trypanosoma brucei gambiense

pH Range

pH Minimum pH Maximum Comment Organism
5 7 the enzyme shows 45-100% over a wide range of pH (5.0-7.0) at 37°C. Activity is almost zero up to pH 4.5, but declines relatively slower in the alkaline side Trypanosoma brucei gambiense