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Literature summary for 2.7.1.32 extracted from
- Choline kinase from brewers yeast. Partial purification, properties, and kinetic mechanism (1973), J. Biol. Chem., 248, 2364-2371.
General Stability
| General Stability | Organism |
|---|---|
| choline, ATP, bovine serum albumin and changes in ionic strength do not contribute to the stability of choline kinase | Saccharomyces cerevisiae |
| loss of activity upon freezing and thawing | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Saccharomyces cerevisiae |  |
| Acetylcholine | - |
Saccharomyces cerevisiae | |
| ADP | - |
Saccharomyces cerevisiae | |
| N-ethylmaleimide | - |
Saccharomyces cerevisiae | |
| phosphorylcholine | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
| 0.14 | - |
ATP | pH 8.5, 25°C | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + choline = ADP + phosphocholine | random bi-bi mechanism | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 10 mM Tris actetate, pH 7.2, 13 mM 2-mercaptoethanol, 1 mM EGTA, 15 mM MgCl2, stable for 1 week | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + choline | - |
Saccharomyces cerevisiae | ADP + O-phosphocholine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
40 min, without Mg2+, 80% loss of activity, + 15 mM Mg2+, 9% loss of activity | Saccharomyces cerevisiae |
| 39 | - |
10 min, 15 mM Mg2+, 50% loss of activity | Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
or below, 2-3 h, 90% or more loss of activity, crude enzyme extract | Saccharomyces cerevisiae |
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