Literature summary for 2.7.1.36 extracted from
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
cysteine |
or to a lesser extent other SH-compounds required |
Oryctolagus cuniculus |
|
phosphate |
required for activity |
Oryctolagus cuniculus |
|
General Stability
General Stability |
Organism |
almost complete loss of activity after dialysis against 1000 volumes of water, 2-mercaptoethanol, EDTA or glutathione |
Oryctolagus cuniculus |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
p-chloromercuribenzoate |
activity can be almost completely restored by incubation with an excess of cysteine |
Oryctolagus cuniculus |
|
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
5.1 |
- |
DL-mevalonate |
pH 7.0, 35°C |
Oryctolagus cuniculus |
|
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Mg2+ |
activates |
Oryctolagus cuniculus |
|
Mg2+ |
Mn2+ or Mg2+ required |
Oryctolagus cuniculus |
|
Mg2+ |
most active divalent cation |
Oryctolagus cuniculus |
|
Mn2+ |
divalent cation required for activity |
Oryctolagus cuniculus |
|
Mn2+ |
maximal activation at approx. 0.0013 mM |
Oryctolagus cuniculus |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Oryctolagus cuniculus |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
ammonium sulfate, protamine sulfate, ammonium sulfate, DEAE-cellulose |
Oryctolagus cuniculus |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
liver |
- |
Oryctolagus cuniculus |
- |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
0.11 |
- |
- |
Oryctolagus cuniculus |
Storage Stability
Storage Stability |
Organism |
-20°C, 50% loss of activity after 1 month |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
ATP + mevalonate |
ATP is the most effective phosphate donor |
Oryctolagus cuniculus |
ADP + phosphomevalonate |
- |
? |
|
ATP + mevalonate |
no activity with ADP, GTP and UTP |
Oryctolagus cuniculus |
ADP + phosphomevalonate |
- |
? |
|
ITP + mevalonate |
36% of activity with ATP |
Oryctolagus cuniculus |
IDP + phosphomevalonate |
- |
? |
|
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
35 |
37 |
assay at |
Oryctolagus cuniculus |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
7 |
8 |
- |
Oryctolagus cuniculus |