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Literature summary for 2.7.1.36 extracted from
- Cloning, expression, and purification of His-tagged rat mevalonate kinase (2003), Protein Expr. Purif., 27, 165-170.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged mevalonate kinase in Escherichia coli | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
(R,S)-mevalonate | pH 7.5, 25°C, recombinant mevalonate kinase | Rattus norvegicus |  |
| 0.95 | - |
ATP | pH 7.5, 25°C, recombinant mevalonate kinase | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000, recombinant mevalonate kinase, SDS-PAGE | Rattus norvegicus |
| 84000 | - |
recombinant mevalonate kinase, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged mevalonate kinase, hiTrap column | Rattus norvegicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 32.7 | - |
recombinant mevalonate kinase | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 20 mM potassium phosphate, pH 7.5, 5% glycerol, 5 mM 2-mercaptoethanol, 1 month, no loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + mevalonate | - |
Rattus norvegicus | ADP + phosphomevalonate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42000, recombinant mevalonate kinase, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | recombinant mevalonate kinase | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | at least 80% of maximal activity over the whole range, recombinant mevalonate kinase | Rattus norvegicus |
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