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Literature summary for 2.7.1.36 extracted from

  • Nyati, P.; Rivera-Perez, C.; Noriega, F.G.
    Negative feedbacks by isoprenoids on a mevalonate kinase expressed in the corpora allata of mosquitoes (2015), PLoS ONE, 10, e0143107.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Aedes aegypti

Inhibitors

Inhibitors Comment Organism Structure
farnesyl diphosphate
-
Aedes aegypti
geranyl diphosphate
-
Aedes aegypti
geranylgeranyl diphosphate
-
Aedes aegypti
additional information not inhibited by by isopentenyl diphosphate and dimethylallyl diphosphate Aedes aegypti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
(R)-mevalonate at pH 7.5, temperature not specified in the publication Aedes aegypti
0.14
-
ATP at pH 7.5, temperature not specified in the publication Aedes aegypti

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Co2+ enhances enzyme to a lesser degree than Mg2+ Aedes aegypti
Mg2+ in the presence of Mg2+, the enzyme has higher affinity for (R)-mevalonate than ATP Aedes aegypti
Mn2+ Mn2+ enhances enzyme to a lesser degree than Mg2+ Aedes aegypti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43270
-
x * 43270, calculated from amino acid sequence Aedes aegypti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-mevalonate Aedes aegypti 100% activity with ATP ADP + (R)-5-phosphomevalonate
-
?

Organism

Organism UniProt Comment Textmining
Aedes aegypti
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap affinity column chromatography Aedes aegypti

Source Tissue

Source Tissue Comment Organism Textmining
corpus allatum
-
Aedes aegypti
-
thorax
-
Aedes aegypti
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-mevalonate 100% activity with ATP Aedes aegypti ADP + (R)-5-phosphomevalonate
-
?
CTP + (R)-mevalonate 5% activity with GTP compared to ATP Aedes aegypti CDP + (R)-5-phosphomevalonate
-
?
GTP + (R)-mevalonate 15% activity with GTP compared to ATP Aedes aegypti GDP + (R)-5-phosphomevalonate
-
?
TTP + (R)-mevalonate 11% activity with GTP compared to ATP Aedes aegypti TDP + (R)-5-phosphomevalonate
-
?

Subunits

Subunits Comment Organism
? x * 43270, calculated from amino acid sequence Aedes aegypti

Synonyms

Synonyms Comment Organism
MVK
-
Aedes aegypti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.1
-
ATP at pH 7.5, temperature not specified in the publication Aedes aegypti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Aedes aegypti

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00044
-
farnesyl diphosphate at pH 7.5, temperature not specified in the publication Aedes aegypti
0.00055
-
geranyl diphosphate at pH 7.5, temperature not specified in the publication Aedes aegypti
0.00093
-
geranylgeranyl diphosphate at pH 7.5, temperature not specified in the publication Aedes aegypti

pI Value

Organism Comment pI Value Maximum pI Value
Aedes aegypti calculated from amino acid sequence
-
5.8