Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Aedes aegypti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
farnesyl diphosphate | - |
Aedes aegypti | |
geranyl diphosphate | - |
Aedes aegypti | |
geranylgeranyl diphosphate | - |
Aedes aegypti | |
additional information | not inhibited by by isopentenyl diphosphate and dimethylallyl diphosphate | Aedes aegypti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
(R)-mevalonate | at pH 7.5, temperature not specified in the publication | Aedes aegypti | |
0.14 | - |
ATP | at pH 7.5, temperature not specified in the publication | Aedes aegypti |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Co2+ enhances enzyme to a lesser degree than Mg2+ | Aedes aegypti | |
Mg2+ | in the presence of Mg2+, the enzyme has higher affinity for (R)-mevalonate than ATP | Aedes aegypti | |
Mn2+ | Mn2+ enhances enzyme to a lesser degree than Mg2+ | Aedes aegypti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43270 | - |
x * 43270, calculated from amino acid sequence | Aedes aegypti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | Aedes aegypti | 100% activity with ATP | ADP + (R)-5-phosphomevalonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aedes aegypti | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap affinity column chromatography | Aedes aegypti |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
corpus allatum | - |
Aedes aegypti | - |
thorax | - |
Aedes aegypti | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | 100% activity with ATP | Aedes aegypti | ADP + (R)-5-phosphomevalonate | - |
? | |
CTP + (R)-mevalonate | 5% activity with GTP compared to ATP | Aedes aegypti | CDP + (R)-5-phosphomevalonate | - |
? | |
GTP + (R)-mevalonate | 15% activity with GTP compared to ATP | Aedes aegypti | GDP + (R)-5-phosphomevalonate | - |
? | |
TTP + (R)-mevalonate | 11% activity with GTP compared to ATP | Aedes aegypti | TDP + (R)-5-phosphomevalonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43270, calculated from amino acid sequence | Aedes aegypti |
Synonyms | Comment | Organism |
---|---|---|
MVK | - |
Aedes aegypti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.1 | - |
ATP | at pH 7.5, temperature not specified in the publication | Aedes aegypti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Aedes aegypti |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00044 | - |
farnesyl diphosphate | at pH 7.5, temperature not specified in the publication | Aedes aegypti | |
0.00055 | - |
geranyl diphosphate | at pH 7.5, temperature not specified in the publication | Aedes aegypti | |
0.00093 | - |
geranylgeranyl diphosphate | at pH 7.5, temperature not specified in the publication | Aedes aegypti |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aedes aegypti | calculated from amino acid sequence | - |
5.8 |