Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | 100 mM NH4Cl results in 35% of the activation compared to 100 mM K+ | Pisum sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-hydroxy-DL-Lys | 5 mM, 92% inhibition | Pisum sativum | |
L-Arg | 10 mM, 13% inhibition | Pisum sativum | |
L-Cys | 10 mM, 25% inhibition | Pisum sativum | |
L-Ile | 7.5 mM, 90% inhibition | Pisum sativum | |
L-Leu | 10 mM, 20% inhibition | Pisum sativum | |
L-Lys | 10 mM, 35% inhibition | Pisum sativum | |
L-Met | 10 mM, 17% inhibition | Pisum sativum | |
L-Orn | 10 mM, 64% inhibition | Pisum sativum | |
L-Ser | 10 mM, 10% inhibition | Pisum sativum | |
L-Thr | 10 mM, 18% inhibition | Pisum sativum | |
L-Val | 16 mM, 86% inhibition | Pisum sativum | |
O-Phospho-DL-homoserine | 10 mM, 91% inhibition | Pisum sativum | |
S-adenosyl-L-methionine | 10 mM, 92% inhibition | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
ATP | pH 8.5 | Pisum sativum | |
6.7 | - |
L-homoserine | pH 8.5 | Pisum sativum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | ineffective | Pisum sativum | |
K+ | absolute requirement for monovalent cation. Km: 21 mM | Pisum sativum | |
Mg2+ | absolute requirement for divalent cation, Mg2+ or Mn2+ | Pisum sativum | |
Mn2+ | absolute requirement for divalent cation, Mg2+ or Mn2+ | Pisum sativum | |
Zn2+ | ineffective | Pisum sativum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
2 peaks of MW 120000 Da and 240000 Da are detected by gel filtration | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-homoserine | Pisum sativum | the enzyme catalyzes a reaction common to synthesis of Thr, Ile and Met in plants | ADP + O-phospho-L-homoserine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
L. cv. Pillert Fenomen | - |
Purification (Comment) | Organism |
---|---|
partial | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Pisum sativum | - |
shoot | green | Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.024 | - |
- |
Pisum sativum |
Storage Stability | Organism |
---|---|
-25°C, purified enzyme is stable for at least 1 month | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-homoserine | - |
Pisum sativum | ADP + O-phospho-L-homoserine | - |
? | |
ATP + L-homoserine | the enzyme catalyzes a reaction common to synthesis of Thr, Ile and Met in plants | Pisum sativum | ADP + O-phospho-L-homoserine | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
homoserine and MgATP2- do not protect against heat inactivation | Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pisum sativum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.1 | 9 | the enzyme shows good activity in Tris-HCl buffer from pH 8.1 to pH 9.0 | Pisum sativum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
L-Val | pH 8.5 | Pisum sativum | |
0.9 | - |
L-Ile | pH 8.5 | Pisum sativum | |
4.3 | - |
L-Orn | pH 8.5 | Pisum sativum |