Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-diphosphate | activation | Busycotypus canaliculatum | |
D-fructose 2,6-diphosphate | activation | Busycotypus canaliculatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | not | Busycotypus canaliculatum | |
3-phosphoglycerate | not | Busycotypus canaliculatum | |
ADP | at high concentrations; fructose 1,6-diphosphate partially restores | Busycotypus canaliculatum | |
AMP | MgAMP | Busycotypus canaliculatum | |
arginine phosphate | allosteric inhibitor; fructose 1,6-diphosphate restores | Busycotypus canaliculatum | |
ATP | MgATP2- | Busycotypus canaliculatum | |
citrate | not | Busycotypus canaliculatum | |
glucose 6-phosphate | not | Busycotypus canaliculatum | |
isocitrate | not | Busycotypus canaliculatum | |
L-alanine | allosteric inhibitor; fructose 1,6-diphosphate restores | Busycotypus canaliculatum | |
L-lactate | - |
Busycotypus canaliculatum | |
L-phenylalanine | alanine and fructose 1,6-diphosphate protect, kinetics; allosteric inhibitor; pH-dependent | Busycotypus canaliculatum | |
malate | not | Busycotypus canaliculatum | |
MgATP2- | allosteric inhibitor; fructose 1,6-diphosphate restores | Busycotypus canaliculatum | |
additional information | no inhibition by acetyl-CoA, NADP+, succinate, glycerol 1-phosphate, fructose 6-phosphate, D-octopine, meso-alanopine, NH4Cl, Arg, Gly, taurine, creatine phosphate | Busycotypus canaliculatum | |
oxaloacetate | not | Busycotypus canaliculatum | |
phosphate | not | Busycotypus canaliculatum | |
proline | allosteric inhibitor, fructose 1,6-diphosphate restores | Busycotypus canaliculatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic properties | Busycotypus canaliculatum | |
0.2 | 0.35 | ADP | pH 7.0, 20°C | Busycotypus canaliculatum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Busycotypus canaliculatum | |
Mg2+ | - |
Busycotypus canaliculatum | |
Mn2+ | can partially replace Mg2+ | Busycotypus canaliculatum | |
NH4+ | requirement | Busycotypus canaliculatum | |
NH4+ | can replace K+ | Busycotypus canaliculatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64400 | - |
4 * 64400, PK-aerobic, SDS-PAGE | Busycotypus canaliculatum |
232600 | - |
PK-anoxic, gel filtration | Busycotypus canaliculatum |
237500 | - |
PK-aerobic, gel filtration | Busycotypus canaliculatum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Busycotypus canaliculatum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
aerobic and anoxic variants, i.e. isozymes PK-aerobic and PK-anoxic | Busycotypus canaliculatum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | radular retractor muscle | Busycotypus canaliculatum | - |
muscle | red muscle | Busycotypus canaliculatum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Busycotypus canaliculatum |
368 | - |
isozyme PK-aerobic, pH 7.0, 20°C | Busycotypus canaliculatum |
Storage Stability | Organism |
---|---|
4°C, in 25 mM imidazole-HCl buffer, pH 7.1, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 M sucrose and 0.04% NaN3, concentrated by dialysis against solid polyethylene glycol, at least 4 weeks, isozyme PK-aerobic | Busycotypus canaliculatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Busycotypus canaliculatum | ATP + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 64400, PK-aerobic, SDS-PAGE | Busycotypus canaliculatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-dependence of phosphoenolpyruvate affinity | Busycotypus canaliculatum |
7 | - |
broad | Busycotypus canaliculatum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | about 70% of maximal activity at pH 6 and about half-maximal activity at pH 8 | Busycotypus canaliculatum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Busycotypus canaliculatum | isozyme PK-anoxic, isoelectric focusing | - |
5.42 |
Busycotypus canaliculatum | isozyme PK-aerobic, isoelectric focusing | - |
5.81 |