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Literature summary for 2.7.1.40 extracted from
- Partial purification and properties of pyruvate kinase and its regulatory role during lipid accumulation by the oleaginous yeast Rhodosporidium toruloides CBS 14 (1985), Can. J. Microbiol., 31, 479-484.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 1,6-diphosphate | activation | Rhodotorula toruloides |  |
| glutamic acid | activation | Rhodotorula toruloides | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | not | Rhodotorula toruloides | |
| ATP | ADP alleviates; kinetics | Rhodotorula toruloides | |
| citrate | - |
Rhodotorula toruloides | |
| CTP | - |
Rhodotorula toruloides | |
| GTP | - |
Rhodotorula toruloides | |
| isocitrate | - |
Rhodotorula toruloides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
ADP | - |
Rhodotorula toruloides | |
| 0.4 | 0.57 | phosphoenolpyruvate | - |
Rhodotorula toruloides | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | requirement | Rhodotorula toruloides | |
| K+ | Km-value 1.6 mM | Rhodotorula toruloides | |
| Na+ | not | Rhodotorula toruloides | |
| NH4+ | can replace K+ | Rhodotorula toruloides | |
| NH4+ | activation | Rhodotorula toruloides | |
| NH4+ | kinetics | Rhodotorula toruloides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 190000 | - |
gel filtration | Rhodotorula toruloides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula toruloides | - |
- |
- |
| Rhodotorula toruloides CBS 14 | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Rhodotorula toruloides | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhodotorula toruloides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | - |
Rhodotorula toruloides | ATP + pyruvate | - |
? | |
| ADP + phosphoenolpyruvate | - |
Rhodotorula toruloides CBS 14 | ATP + pyruvate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rhodotorula toruloides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 8.2 | - |
Rhodotorula toruloides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| AMP | not | Rhodotorula toruloides | |
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Release 2023.1 (January 2023)
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