Literature summary for 2.7.1.40 extracted from

Ernest, I.; Callens, M.; Uttaro, A.D.; Chevalier, N.; Opperdoes, F.R.; Muirhead, H.; Michels, P.A.
Pyruvate kinase of Trypanosoma brucei: overexpression, purification, and functional characterization of wild-type and mutated enzyme (1998), Protein Expr. Purif., 13, 373-382.

Search for more literature for 2.7.1.40

Activating Compound

Activating Compound	Comment	Organism	Structure
D-fructose diphosphate	allosteric effector	Trypanosoma brucei

Protein Variants

Protein Variants	Comment	Organism
F463V	reduced affinity for fructose 1,6-diphosphate and fructose 2,6-diphosphate	Trypanosoma brucei
R22G	strongly reduced affinity for fructose 1,6-diphosphate and fructose 2,6-diphosphate	Trypanosoma brucei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.098	0.1	GDP	pH 7.5, 25°C	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + phosphoenolpyruvate	-	Trypanosoma brucei	ATP + pyruvate	-	?
GDP + phosphoenolpyruvate	-	Trypanosoma brucei	GTP + pyruvate	-	?

pI Value

Organism	Comment	pI Value Maximum	pI Value
Trypanosoma brucei	isoelectric focusing	-	7.95

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Release 2023.1 (January 2023)