Literature summary for 2.7.1.40 extracted from

Andre, C.; Froehlich, J.E.; Moll, M.R.; Benning, C.
A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis (2007), Plant Cell, 19, 2006-2022.

Search for more literature for 2.7.1.40

Activating Compound

Activating Compound	Comment	Organism	Structure
6-phosphogluconate	192% of activity at 0.05 mM	Arabidopsis thaliana
additional information	not activated by 6-phosphogluconate	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
K314L	no increased activity at pH 7.0	Arabidopsis thaliana
K325L	no increased activity at pH 7.0	Arabidopsis thaliana
K344L	no increased activity at pH 7.0	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
AMP	1 mM, 77% of activity remaining	Arabidopsis thaliana
ATP	1 mM, 77% of activity remaining; 1 mM, 87% of activity remaining	Arabidopsis thaliana
glyoxylate	5 mM, 79% of activity remaining; 5 mM, 83% of activity remaining	Arabidopsis thaliana
iso-citrate	10 mM, 70% of activity remaining; 10 mM, 75% of activity remaining	Arabidopsis thaliana
L-glutamate	5 mM, 30% of activity remaining, IC50: 2.1 mM, IC50: 6.2 mM; 5 mM, 59% of activity remaining, IC50: 6.2 mM	Arabidopsis thaliana
additional information	not inhibited by 6-phosphogluconate, dithiothreitol or sodium tetrathionate; not inhibited by AMP, dithiothreitol or sodium tetrathionate	Arabidopsis thaliana
oxalate	0.2 mM, 50% of activity remaining; 0.2 mM, 71% of activity remaining, IC50: 0.41 mM	Arabidopsis thaliana
oxaloacetate	2 mM, 82% of activity remaining; 2 mM, 88% of activity remaining	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plastid	-	Arabidopsis thaliana	9536	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	strict requirement	Arabidopsis thaliana
Mg2+	strict requirement	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
57000	-	4 * 60000 + 4 * 57000, gel filtration	Arabidopsis thaliana
60000	-	4 * 60000 + 4 * 57000, gel filtration	Arabidopsis thaliana
463000	-	gel filtration	Arabidopsis thaliana
476000	-	gel filtration	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin chromatography	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + phosphoenolpyruvate	-	Arabidopsis thaliana	ATP + pyruvate	-	?

Subunits

Subunits	Comment	Organism
octamer	4 * 60000 + 4 * 57000, gel filtration	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
PK-alphabeta1	-	Arabidopsis thaliana
PK-alphabeta2	-	Arabidopsis thaliana
PKp	-	Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	55	completely inactivated after 3 min of incubation at 50 to 55°C	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
ADP	pyruvate kinase activity is not observed in the absence of ADP	Arabidopsis thaliana

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.41	-	0.2 mM, 71% of activity remaining, IC50: 0.41 mM	Arabidopsis thaliana	oxalate
6.2	-	5 mM, 30% of activity remaining, IC50: 2.1 mM, IC50: 6.2 mM	Arabidopsis thaliana	L-glutamate
6.2	-	5 mM, 59% of activity remaining, IC50: 6.2 mM	Arabidopsis thaliana	L-glutamate

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Release 2023.1 (January 2023)