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Literature summary for 2.7.1.40 extracted from

  • Saito, T.; Nishi, M.; Lim, M.I.; Wu, B.; Maeda, T.; Hashimoto, H.; Takeuchi, T.; Roos, D.S.; Asai, T.
    A novel GDP-dependent pyruvate kinase isozyme from Toxoplasma gondii localizes to both the apicoplast and the mitochondrion (2008), J. Biol. Chem., 283, 14041-14052.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.40

Activating Compound

Activating Compound Comment Organism Structure
additional information no allosteric or regulatory effects are observed in the presence of D-fructose 1,6-diphosphate, D-fructose 2,6-diphosphate, D-glucose 6-phosphate, D-ribose 5-phosphate, AMP, ATP, ITP, AMP, L-His, L-Ser, L-Ala, L-Glu, Gln, L-Thr, L-Met, Gly, L-le, L-Asn, L-Cys, L-Pro, L-Arg, L-Lys, L-Phe, L-Trp, L-Leu, L-Asp, L-Val (1 mM each), L-Tyr (0.5 mM), or 0.1 mM acetyl-CoA Toxoplasma gondii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Toxoplasma gondii

General Stability

General Stability Organism
the enzyme activity remains stable after one freeze-thaw cycle Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
GDP
-
 Toxoplasma gondii
GTP 0.1 mM GTP reduces the Vmax by 10% Toxoplasma gondii
IDP
-
 Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0544
-
 GDP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
0.116
-
 phosphoenolpyruvate PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
0.193
-
 IDP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
7.99
-
 ADP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
apicoplast
-
 Toxoplasma gondii 20011
-
mitochondrion
-
 Toxoplasma gondii 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information catalytic activity is independent of K+ Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
 purified recombinant protein Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyo Pearl 650 column chromatography and glutathione Sepharose column chromatography Toxoplasma gondii

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite
-
 Toxoplasma gondii
-

Storage Stability

Storage Stability Organism
4°C, 20 mM Tris-HCl, pH 7.0, and 30% glycerol, 1 week, remains stable Toxoplasma gondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
 Toxoplasma gondii ATP + pyruvate
-
 ?
GDP + phosphoenolpyruvate preference for GDP Toxoplasma gondii GTP + pyruvate
-
 ?
IDP + phosphoenolpyruvate
-
 Toxoplasma gondii ITP + pyruvate
-
 ?

Synonyms

Synonyms Comment Organism
PyKII
-
 Toxoplasma gondii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
47.6
-
 ADP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
110
-
 GDP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
131
-
 IDP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Toxoplasma gondii

pH Range

pH Minimum pH Maximum Comment Organism
8 9.5 more than 80% of the maximal activity is observed between pH 8.0 and pH 9.5 Toxoplasma gondii

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 at pH 7.0, the activity of PyKII is 50% of the maximal activity Toxoplasma gondii

Cofactor

Cofactor Comment Organism Structure
ADP
-
 Toxoplasma gondii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6
-
 ADP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
681
-
 IDP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
2020
-
 GDP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii