Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.67 extracted from

  • Hou, W.M.; Zhang, Z.L.; Tai, H.H.
    Purification and characterization of phosphatidylinositol kinase from porcine liver microsomes (1988), Biochim. Biophys. Acta, 959, 67-75.
    View publication on PubMed
Search for more literature for 2.7.1.67

Inhibitors

Inhibitors Comment Organism Structure
adenosine IC50: 0.07 mM. 1 mM, 93% inhibition Sus scrofa
ADP IC50: 0.12 mM. 1 mM, 92% inhibition Sus scrofa
AMP 1 mM, 48% inhibition Sus scrofa
Ca2+ inhibits Mg2+-stimulated activity, IC50: 0.4 mM Sus scrofa
deoxycholate 10 mM, 35% inhibition Sus scrofa
GDP 1 mM, 27% inhibition Sus scrofa
GMP 1 mM, 20% inhibition Sus scrofa
GTP 1 mM, 24% inhibition Sus scrofa
guanosine 1 mM, 50% inhibition Sus scrofa
Lubrol Px 1%, 72% inhibition Sus scrofa
NEM 0.1 mM, 42% inhibition Sus scrofa
PCMB 0.1 mM, 98% inhibition Sus scrofa
phosphatidylethanolamine 0.1 mM, 24% inhibition Sus scrofa
phosphatidylglycerol 0.1 mM, 63% inhibition Sus scrofa
phosphatidylserine 0.1 mM, 33% inhibition Sus scrofa
quercetin IC50: 0.1 mM Sus scrofa
Sodium mersalyl 0.1 mM, 78% inhibition Sus scrofa
Triton X-100 0.2%, 11% inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 ATP pH 7.5, 30°C Sus scrofa
0.12
-
 phosphatidylinositol pH 7.5, 30°C Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Sus scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+ Sus scrofa
Fe2+ divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+ Sus scrofa
Fe3+ divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+ Sus scrofa
Mg2+ divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+ Sus scrofa
Mn2+ divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+ Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.824
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol
-
 Sus scrofa ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Sus scrofa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.07
-
 IC50: 0.07 mM. 1 mM, 93% inhibition Sus scrofa adenosine
0.1
-
 IC50: 0.1 mM Sus scrofa quercetin
0.12
-
 IC50: 0.12 mM. 1 mM, 92% inhibition Sus scrofa ADP
0.4
-
 inhibits Mg2+-stimulated activity, IC50: 0.4 mM Sus scrofa Ca2+