General Stability | Organism |
---|---|
bovine serum albumin does not stabilize | Mus musculus |
protamine treatment, MnCl2-treatment or ammonium sulfate fractionation inactivates during purification | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cytidine | not | Mus musculus | |
cytosine arabinoside | deoxycytidine as substrate | Mus musculus | |
dAMP | not | Mus musculus | |
dCDP | - |
Mus musculus | |
dCMP | - |
Mus musculus | |
dCTP | strong, pH-dependent | Mus musculus | |
dGMP | not | Mus musculus | |
dinitrophenol | weak | Mus musculus | |
dioxane | - |
Mus musculus | |
dUMP | not | Mus musculus | |
HgCl2 | - |
Mus musculus | |
IAA | weak | Mus musculus | |
additional information | little or no inhibition by deoxyuridine; no inhibition by TTP; no inhibition by uridine, thymidine; relatively insensitive to SH-inhibitors and detergents | Mus musculus | |
N-ethylmaleimide | weak | Mus musculus | |
SDS | - |
Mus musculus | |
Urea | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Mus musculus | |
Ca2+ | can replace Mn2+ or Mg2+ to some extent | Mus musculus | |
Fe2+ | activation | Mus musculus | |
Fe2+ | can replace Mn2+ or Mg2+ to some extent | Mus musculus | |
Mg2+ | requirement, 2 mM | Mus musculus | |
Mn2+ | requirement | Mus musculus | |
Mn2+ | 0.5 mM | Mus musculus | |
additional information | no activation by Ba2+, Cu2+ | Mus musculus | |
Zn2+ | not | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leukemia cell | L1210 | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0048 | - |
37°C, pH 7.0 | Mus musculus |
Storage Stability | Organism |
---|---|
-20°C, 3 months | Mus musculus |
4°C, at least 1 week | Mus musculus |
glycerol, bovine serum albumin, 2-mercaptoethanol or EDTA does not enhance storage stability | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxycytidine | best phosphate donor | Mus musculus | ADP + 5'-dCMP | - |
? | |
NTP + cytosine arabinoside | - |
Mus musculus | NDP + cytosine arabinoside 5'-monophosphate | - |
? | |
NTP + deoxycytidine | no substrate: phosphate | Mus musculus | NDP + dCMP | - |
? | |
NTP + deoxycytidine | enzyme shows little specificity towards phosphate donor | Mus musculus | NDP + dCMP | - |
? | |
UTP + 2'-deoxycytidine | best phosphate donor at low substrate levels | Mus musculus | UDP + 2'-deoxy-CMP | - |
? | |
UTP + 2'-deoxycytidine | as good as CTP, GTP or dATP | Mus musculus | UDP + 2'-deoxy-CMP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | 37 | moderately sensitive to variations of incubation temperature | Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, 10% loss of activity | Mus musculus |
60 | - |
10 min, about 65% loss of activity | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | broad | Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | maximal activity at pH 5 and about half-maximal activity at pH 10 | Mus musculus |