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Literature summary for 2.7.1.74 extracted from
- Properties of deoxycytidine kinase partially purified from L1210 cells (1968), J. Biol. Chem., 243, 4739-4744.
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin does not stabilize | Mus musculus |
| protamine treatment, MnCl2-treatment or ammonium sulfate fractionation inactivates during purification | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cytidine | not | Mus musculus |  |
| cytosine arabinoside | deoxycytidine as substrate | Mus musculus | |
| dAMP | not | Mus musculus | |
| dCDP | - |
Mus musculus | |
| dCMP | - |
Mus musculus | |
| dCTP | strong, pH-dependent | Mus musculus | |
| dGMP | not | Mus musculus | |
| dinitrophenol | weak | Mus musculus | |
| dioxane | - |
Mus musculus | |
| dUMP | not | Mus musculus | |
| HgCl2 | - |
Mus musculus | |
| IAA | weak | Mus musculus | |
| additional information | little or no inhibition by deoxyuridine; no inhibition by TTP; no inhibition by uridine, thymidine; relatively insensitive to SH-inhibitors and detergents | Mus musculus | |
| N-ethylmaleimide | weak | Mus musculus | |
| SDS | - |
Mus musculus | |
| Urea | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic study | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation | Mus musculus | |
| Ca2+ | can replace Mn2+ or Mg2+ to some extent | Mus musculus | |
| Fe2+ | activation | Mus musculus | |
| Fe2+ | can replace Mn2+ or Mg2+ to some extent | Mus musculus | |
| Mg2+ | requirement, 2 mM | Mus musculus | |
| Mn2+ | requirement | Mus musculus | |
| Mn2+ | 0.5 mM | Mus musculus | |
| additional information | no activation by Ba2+, Cu2+ | Mus musculus | |
| Zn2+ | not | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leukemia cell | L1210 | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0048 | - |
37°C, pH 7.0 | Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 3 months | Mus musculus |
| 4°C, at least 1 week | Mus musculus |
| glycerol, bovine serum albumin, 2-mercaptoethanol or EDTA does not enhance storage stability | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxycytidine | best phosphate donor | Mus musculus | ADP + 5'-dCMP | - |
? | |
| NTP + cytosine arabinoside | - |
Mus musculus | NDP + cytosine arabinoside 5'-monophosphate | - |
? | |
| NTP + deoxycytidine | no substrate: phosphate | Mus musculus | NDP + dCMP | - |
? | |
| NTP + deoxycytidine | enzyme shows little specificity towards phosphate donor | Mus musculus | NDP + dCMP | - |
? | |
| UTP + 2'-deoxycytidine | best phosphate donor at low substrate levels | Mus musculus | UDP + 2'-deoxy-CMP | - |
? | |
| UTP + 2'-deoxycytidine | as good as CTP, GTP or dATP | Mus musculus | UDP + 2'-deoxy-CMP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 37 | moderately sensitive to variations of incubation temperature | Mus musculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
10 min, 10% loss of activity | Mus musculus |
| 60 | - |
10 min, about 65% loss of activity | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | broad | Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | maximal activity at pH 5 and about half-maximal activity at pH 10 | Mus musculus |
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