Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | - |
Rattus norvegicus | |
spermidine | little influence | Rattus norvegicus | |
spermine | little influence | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Rattus norvegicus | |
AgNO3 | - |
Rattus norvegicus | |
Cu2+ | inhibits in presence of MgCl2 | Rattus norvegicus | |
Deoxyribonucleoside triphosphates | - |
Rattus norvegicus | |
Dextran sulfate | strong inhibition | Rattus norvegicus | |
heparin | strong inhibition | Rattus norvegicus | |
iodoacetate | - |
Rattus norvegicus | |
p-chloromercuribenzoate | - |
Rattus norvegicus | |
Ribonucleoside triphosphates | - |
Rattus norvegicus | |
sulfate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
ATP | pH 5.5, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Rattus norvegicus | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective | Rattus norvegicus | |
Co2+ | Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective | Rattus norvegicus | |
divalent cations | absolutely required | Rattus norvegicus | |
divalent cations | Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective, descending order | Rattus norvegicus | |
Mg2+ | Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective | Rattus norvegicus | |
Mn2+ | Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective | Rattus norvegicus | |
Ni2+ | Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective in activation | Rattus norvegicus | |
Zn2+ | Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | Rattus norvegicus | - |
ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | Rattus norvegicus | - |
ADP + 5'-phospho-DNA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, 960fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.006 | - |
partially purified enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | - |
Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | - |
Rattus norvegicus | ADP + 5'-phospho-DNA | - |
r | |
ATP + 5'-dephospho-DNA | double stranded DNA | Rattus norvegicus | ADP + 5'-phospho-DNA | UDP, ADP, GDP and CDP support the reverse reaction | r | |
ATP + 5'-dephospho-DNA | large DNA fragments released by nuclease treatment | Rattus norvegicus | ADP + 5'-phospho-DNA | UDP, ADP, GDP and CDP support the reverse reaction | r | |
ATP + 5'-dephospho-DNA | single stranded DNA | Rattus norvegicus | ADP + 5'-phospho-DNA | UDP, ADP, GDP and CDP support the reverse reaction | r | |
ATP + 5'-dephospho-DNA | native and heat denatured DNA | Rattus norvegicus | ADP + 5'-phospho-DNA | UDP, ADP, GDP and CDP support the reverse reaction | r | |
ATP + 5'-dephospho-RNA | no activity | Rattus norvegicus | ADP + 5'-phospho-RNA | - |
? | |
ATP + synthetic oligonucleotide | no activity with low molecular weight oligonucleotides | Rattus norvegicus | ADP + oligonucleotide 5'-phosphate | - |
? | |
ATP + synthetic oligonucleotide | oligodeoxynucleotides of chain length above 10-12 residues | Rattus norvegicus | ADP + oligonucleotide 5'-phosphate | - |
? | |
CTP + 5'-dephospho-DNA | UDP, ADP, GDP and CDP support the reverse reaction | Rattus norvegicus | CDP + 5'-phospho-DNA | - |
r | |
GTP + 5'-dephospho-DNA | UDP, ADP, GDP and CDP support the reverse reaction | Rattus norvegicus | GDP + 5'-phospho-DNA | - |
r | |
UTP + 5'-dephospho-DNA | UDP, ADP, GDP and CDP support the reverse reaction | Rattus norvegicus | UDP + 5'-phospho-DNA | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
quick inactivation | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
sharp maximum | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6.5 | pH 5: about 50% of activity maximum, pH 6.5: about 35% of activity maximum | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
37°C, unstable below | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Rattus norvegicus | |
CDP | - |
Rattus norvegicus | |
CTP | - |
Rattus norvegicus | |
GDP | - |
Rattus norvegicus | |
GTP | - |
Rattus norvegicus | |
UDP | - |
Rattus norvegicus | |
UTP | - |
Rattus norvegicus |