Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | mixed inhibitor both to NADH and ATP | Arabidopsis thaliana | |
p-chloromercuribenzoate | 0.01 mM, 42% residual activity | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
NADH | pH 7.9, 25°C | Arabidopsis thaliana | |
0.042 | - |
NADH | pH 7.9, 25°C, the specificity constant (vmax/Km) for NADH is 100fold greater than for NAD+ | Arabidopsis thaliana | |
0.062 | - |
ATP | pH 7.9, 25°C | Arabidopsis thaliana | |
0.062 | - |
ATP | pH 7.9, 25°C, cosubstrate NADH | Arabidopsis thaliana | |
0.156 | - |
UTP | pH 7.9, 25°C, cosubstrate NADH | Arabidopsis thaliana | |
0.461 | - |
GTP | pH 7.9, 25°C, cosubstrate NADH | Arabidopsis thaliana | |
0.759 | - |
CTP | pH 7.9, 25°C, cosubstrate NADH | Arabidopsis thaliana | |
2.39 | - |
NAD+ | pH 7.9, 25°C | Arabidopsis thaliana | |
2.39 | - |
NAD+ | pH 7.9, 25°C, the specificity constant (vmax/Km) for NADH is 100fold greater than for NAD+ | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | putative | Arabidopsis thaliana | 5829 | - |
additional information | not: mitochondrial | Arabidopsis thaliana | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | far less active than Mg2+ and Mn2+ | Arabidopsis thaliana | |
Ca2+ | 8% of the activity with Mg2+ | Arabidopsis thaliana | |
Mg2+ | Mn2+ can substitute for Mg2+ to stabilize the phosphoanhydride bonds of ATP but with only 28% of the activity. Km-value Mg2+, 1.16 mM | Arabidopsis thaliana | |
Mg2+ | required, Km-value 1.16 mM | Arabidopsis thaliana | |
Mn2+ | Mn2+ can substitute for Mg2+ to stabilize the phosphoanhydride bonds of ATP but with only 28% of the activity | Arabidopsis thaliana | |
Mn2+ | 28% of the activity with Mg2+ | Arabidopsis thaliana | |
Zn2+ | far less active than Mg2+ and Mn2+ | Arabidopsis thaliana | |
Zn2+ | 4% of the activity with Mg2+ | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Arabidopsis thaliana |
78000 | - |
gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | - |
first discovery in plants | - |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Arabidopsis thaliana | - |
cell suspension culture | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.1 | - |
NAD+ as substrate, pH 7.9, 25°C | Arabidopsis thaliana |
15.6 | - |
pH 7.9, 25°C, substrate NAD+ | Arabidopsis thaliana |
40.1 | - |
NADH as susbtrate, pH 7.9, 25°C | Arabidopsis thaliana |
40.1 | - |
pH 7.9, 25°C, substrate NADH | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | NADH is preferred 100fold over NAD+ | Arabidopsis thaliana | ADP + NADP+ | - |
? | |
ATP + NADH | preferentially phosphorylates reduced NAD+ but can use oxidized NAD+. ATP is preferred phophoryl donor but UTP, GTP, and CTP may substitute with lower activity | Arabidopsis thaliana | ADP + NADPH | - |
? | |
ATP + NADH | NADH is preferred 100fold over NAD+ | Arabidopsis thaliana | ADP + NADPH | - |
? | |
CTP + NADH | ATP is preferred substrate | Arabidopsis thaliana | CDP + NADPH | - |
? | |
GTP + NADH | ATP is preferred substrate | Arabidopsis thaliana | GDP + NADPH | - |
? | |
additional information | diphosphate or polyphosphate is not used as a phosphoryl donor | Arabidopsis thaliana | ? | - |
? | |
additional information | no substrate: diphosphate, polyphosphate | Arabidopsis thaliana | ? | - |
? | |
UTP + NADH | ATP is preferred substrate | Arabidopsis thaliana | UDP + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
diphosphopyridine nucleotide (reduced) kinase | - |
Arabidopsis thaliana |
NAD(H) kinase | - |
Arabidopsis thaliana |
NADHK | - |
Arabidopsis thaliana |
NADK3 | - |
Arabidopsis thaliana |
nicotinamide adenine dinucleotide (reduced) kinase | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
- |
Arabidopsis thaliana |