Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-bisphosphate | shifts pH-optimum for both reaction directions to slightly acidic | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | in the forward reaction only in presence of fructose 2,6-bisphosphate | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters, in the absence or presence of D-fructose 2,6-bisphosphate | Oryza sativa | |
0.139 | 0.21 | D-fructose 1,6-bisphosphate | - |
Oryza sativa | |
0.59 | 1 | phosphate | - |
Oryza sativa | |
3.8 | 10.6 | D-fructose 6-phosphate | with diphosphate | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
1 * 100000, SDS-PAGE | Oryza sativa |
103000 | - |
gel filtration | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | Oryza sativa | - |
phosphate + D-fructose 1,6-bisphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
Japonica cv. Koshihikari | - |
Purification (Comment) | Organism |
---|---|
852fold, to apparent homogeneity | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
48.6 | - |
purified enzyme | Oryza sativa |
Storage Stability | Organism |
---|---|
4°C, 10% loss of activity within 2 weeks | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | - |
Oryza sativa | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Oryza sativa | phosphate + D-fructose 1,6-bisphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, SDS-PAGE | Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
in the presence of D-fructose 2,6-bisphosphate | Oryza sativa |
7.5 | - |
in the presence of D-fructose 2,6-bisphosphate | Oryza sativa |
7.9 | - |
reverse reaction, in absence of D-fructose 2,6-bisphosphate | Oryza sativa |
8.1 | - |
forward reaction, in absence of D-fructose 2,6-bisphosphate | Oryza sativa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.46 | - |
phosphate | versus fructose 6-phosphate, pH 7.5, 25°C, in presence of fructose 2,6-bisphosphate at saturating concentration | Oryza sativa | |
1.53 | - |
phosphate | versus diphosphate, pH 7.5, 25°C, in presence of fructose 2,6-bisphosphate at saturating concentration | Oryza sativa |