Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-bisphosphate | 11fold | Solanum lycopersicum | |
D-fructose 2,6-bisphosphate | forward reaction only | Solanum lycopersicum | |
D-glucose | forward reaction | Solanum lycopersicum | |
D-glucose 1-phosphate | reverse reaction | Solanum lycopersicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction | Solanum lycopersicum | |
Cl- | - |
Solanum lycopersicum | |
Co2+ | complete inhibition in the forward reaction, strong inhibition in the reverse reaction | Solanum lycopersicum | |
diphosphate | - |
Solanum lycopersicum | |
EDTA | - |
Solanum lycopersicum | |
KCl | complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction | Solanum lycopersicum | |
Mn2+ | inhibitory in the forward reaction only, slightly activating in the reverse reaction | Solanum lycopersicum | |
NaCl | complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction | Solanum lycopersicum | |
NH4+ | complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction | Solanum lycopersicum | |
SO42- | gluconeogentic reaction | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | 0.38 | D-fructose 6-phosphate | - |
Solanum lycopersicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | no activation of forward reaction | Solanum lycopersicum | |
Co2+ | no activitaion | Solanum lycopersicum | |
K+ | slightly activating in forward reaction | Solanum lycopersicum | |
Mg2+ | requirement | Solanum lycopersicum | |
Mg2+ | less effective than Mn2+ | Solanum lycopersicum | |
Mn2+ | inhibitory in the forward reaction only, slightly activating in the reverse reaction | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | 62000 | PAGE, gel filtration | Solanum lycopersicum |
64000 | - |
x * 64000 + x * 68000, SDS-PAGE | Solanum lycopersicum |
68000 | - |
x * 64000 + x * 68000, SDS-PAGE | Solanum lycopersicum |
123000 | 136000 | dimer, PAGE, gel filtration | Solanum lycopersicum |
243000 | 262000 | predominant tetrameric form, PAGE, gel filtration, in the presence of diphosphate the oligomer dissociates into monomers, D-fructose 2,6-bisphosphate does not reassociate | Solanum lycopersicum |
425000 | - |
hexamer, PAGE, in the presence of diphosphate the oligomer dissociates into monomers, D-fructose 2,6-bisphosphate does not reassociate | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | Solanum lycopersicum | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
cv. Super First | - |
Purification (Comment) | Organism |
---|---|
partial, 569fold | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | mature green | Solanum lycopersicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.72 | - |
partially purified enzyme | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | - |
Solanum lycopersicum | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
diphosphate + D-fructose 6-phosphate | - |
Solanum lycopersicum | phosphate + D-fructose 1,6-bisphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 64000 + x * 68000, SDS-PAGE | Solanum lycopersicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Solanum lycopersicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
diphosphate | pH 8.0, 25°C | Solanum lycopersicum |