Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, functional expression in Escherichia coli BL21(DE3) as His-tagged protein | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-[N-(2-phenylthioethyl)-N-methylamino]-1-hydroxypropylidene-1,1-bisphosphonate | i.e. CGP 48048, bisphosphonate, synthetic diphosphate analogue; inhibition of growth | Entamoeba histolytica | |
alendronate | bisphosphonate, synthetic diphosphate analogue; inhibition of growth | Entamoeba histolytica | |
clodronate | bisphosphonate, synthetic diphosphate analogue; inhibition of initial growth after inoculation | Entamoeba histolytica | |
EDTA | complete inhibition of growth at 0.005 mM | Entamoeba histolytica | |
etidronate | bisphosphonate, synthetic diphosphate analogue; inhibition of growth | Entamoeba histolytica | |
pamidronate | bisphosphonate, synthetic diphosphate analogue; inhibition of growth | Entamoeba histolytica | |
risedronate | bisphosphonate, synthetic diphosphate analogue; inhibition of growth | Entamoeba histolytica | |
zoledronate | bisphosphonate, synthetic diphosphate analogue; inhibition of initial growth after inoculation | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | 0.017 | diphosphate | native enzyme, pH 7.0, 25°C | Entamoeba histolytica | |
0.018 | - |
diphosphate | recombinant His-tagged enzyme, pH 7.0, 25°C | Entamoeba histolytica | |
0.04 | - |
D-fructose 6-phosphate | native enzyme, pH 7.0, 25°C | Entamoeba histolytica | |
0.045 | - |
D-fructose 6-phosphate | recombinant His-tagged enzyme, pH 7.0, 25°C | Entamoeba histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47580 | - |
x * 47580, DNA sequence determination | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | Q27651 | - |
- |
Purification (Comment) | Organism |
---|---|
native, partial 146fold, further purification leads to inactivation | Entamoeba histolytica |
recombinant His-tagged protein from Escherichia coli | Entamoeba histolytica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Entamoeba histolytica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.136 | - |
purified recombinant His-tagged enzyme | Entamoeba histolytica |
0.24 | - |
partially purified native enzyme | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Entamoeba histolytica | phosphate + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47580, DNA sequence determination | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
PPi-dependent phosphofructokinase | - |
Entamoeba histolytica |
PPi-PFK | - |
Entamoeba histolytica |
pyrophosphate-dependent phosphofructokinase | - |
Entamoeba histolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Entamoeba histolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
zoledronate | pH 7.0, 25°C | Entamoeba histolytica | |
0.05 | - |
3-[N-(2-phenylthioethyl)-N-methylamino]-1-hydroxypropylidene-1,1-bisphosphonate | pH 7.0, 25°C | Entamoeba histolytica | |
0.3 | - |
risedronate | pH 7.0, 25°C | Entamoeba histolytica | |
0.4 | - |
alendronate | pH 7.0, 25°C | Entamoeba histolytica | |
0.56 | - |
etidronate | pH 7.0, 25°C | Entamoeba histolytica | |
85 | - |
clodronate | pH 7.0, 25°C | Entamoeba histolytica |