Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Escherichia coli | Entamoeba histolytica |
Protein Variants | Comment | Organism |
---|---|---|
M249A | site-directed mutagenesis, reduced activity and increased Km-value for fructose 6-phosphate compared to wild-type | Entamoeba histolytica |
M249I | site-directed mutagenesis, highly reduced activity, increased Km-value for fructose 6-phosphate compared to wild-type | Entamoeba histolytica |
M249L | site-directed mutagenesis, highly reduced activity and increased Km-value for fructose 6-phosphate compared to wild-type | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
diphosphate | wild-type, pH 7.2, 30°C | Entamoeba histolytica | |
0.085 | - |
D-fructose 6-phosphate | wild-type, pH 7.2, 30°C | Entamoeba histolytica | |
0.17 | - |
D-fructose 6-phosphate | mutant M249I, pH 7.2, 30°C | Entamoeba histolytica | |
6.8 | - |
D-fructose 6-phosphate | mutant M249A, pH 7.2, 30°C | Entamoeba histolytica | |
4600 | - |
D-fructose 6-phosphate | mutant M249L, pH 7.2, 30°C | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant | Entamoeba histolytica |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate | Met249 plays a critical role in the binding of fructose 6-phosphate and the stabilization of the transition state | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Entamoeba histolytica | phosphate + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | recombinant mutants, SDS-PAGE | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
PPi-PFK | - |
Entamoeba histolytica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
D-fructose 6-phosphate | mutant M249I, pH 7.2, 30°C | Entamoeba histolytica | |
0.42 | - |
D-fructose 6-phosphate | mutant M249L, pH 7.2, 30°C | Entamoeba histolytica | |
92 | - |
D-fructose 6-phosphate | mutant M249A, pH 7.2, 30°C | Entamoeba histolytica | |
325 | - |
D-fructose 6-phosphate | wild-type enzyme, pH 7.2, 30°C | Entamoeba histolytica |