Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.90 extracted from

  • Wang, X.; Deng, Z.; Kemp, R.G.
    An essential methionine residue involved in substrate binding by phosphofructokinases (1998), Biochem. Biophys. Res. Commun., 250, 466-468.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutants in Escherichia coli Entamoeba histolytica

Protein Variants

Protein Variants Comment Organism
M249A site-directed mutagenesis, reduced activity and increased Km-value for fructose 6-phosphate compared to wild-type Entamoeba histolytica
M249I site-directed mutagenesis, highly reduced activity, increased Km-value for fructose 6-phosphate compared to wild-type Entamoeba histolytica
M249L site-directed mutagenesis, highly reduced activity and increased Km-value for fructose 6-phosphate compared to wild-type Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
diphosphate wild-type, pH 7.2, 30°C Entamoeba histolytica
0.085
-
D-fructose 6-phosphate wild-type, pH 7.2, 30°C Entamoeba histolytica
0.17
-
D-fructose 6-phosphate mutant M249I, pH 7.2, 30°C Entamoeba histolytica
6.8
-
D-fructose 6-phosphate mutant M249A, pH 7.2, 30°C Entamoeba histolytica
4600
-
D-fructose 6-phosphate mutant M249L, pH 7.2, 30°C Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant Entamoeba histolytica

Reaction

Reaction Comment Organism Reaction ID
diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate Met249 plays a critical role in the binding of fructose 6-phosphate and the stabilization of the transition state Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Entamoeba histolytica phosphate + D-fructose 1,6-bisphosphate
-
?

Subunits

Subunits Comment Organism
? recombinant mutants, SDS-PAGE Entamoeba histolytica

Synonyms

Synonyms Comment Organism
PPi-PFK
-
Entamoeba histolytica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
D-fructose 6-phosphate mutant M249I, pH 7.2, 30°C Entamoeba histolytica
0.42
-
D-fructose 6-phosphate mutant M249L, pH 7.2, 30°C Entamoeba histolytica
92
-
D-fructose 6-phosphate mutant M249A, pH 7.2, 30°C Entamoeba histolytica
325
-
D-fructose 6-phosphate wild-type enzyme, pH 7.2, 30°C Entamoeba histolytica