Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | complete inhibition at 1 mM | Dictyoglomus thermophilum | |
Fe3+ | 84% inhibition at 0.1 mM | Dictyoglomus thermophilum | |
Mn2+ | 46% inhibition at 0.1 mM | Dictyoglomus thermophilum | |
Se4+ | 75% inhibition at 0.1 mM | Dictyoglomus thermophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
diphosphate | pH 6.0, 50°C | Dictyoglomus thermophilum | |
0.23 | - |
D-fructose 6-phosphate | pH 6.0, 50°C | Dictyoglomus thermophilum | |
2.9 | - |
D-fructose 1,6-bisphosphate | pH 7.2, 50°C | Dictyoglomus thermophilum | |
4.3 | - |
phosphate | pH 7.2, 50°C | Dictyoglomus thermophilum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Dictyoglomus thermophilum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates 120% at 0.1 mM | Dictyoglomus thermophilum | |
Co2+ | - |
Dictyoglomus thermophilum | |
divalent cations | activates 120% at 0.1 mM | Dictyoglomus thermophilum | |
Fe2+ | activates 120% at 0.1 mM | Dictyoglomus thermophilum | |
K+ | activates 140% at 10 mM | Dictyoglomus thermophilum | |
Mg2+ | optimal at 0.5-3.5 mM | Dictyoglomus thermophilum | |
Na+ | activates 135% at 10 mM | Dictyoglomus thermophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Dictyoglomus thermophilum |
65000 | - |
gel filtration | Dictyoglomus thermophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | Dictyoglomus thermophilum | - |
phosphate + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-fructose 6-phosphate | Dictyoglomus thermophilum Rt46 B.1 | - |
phosphate + D-fructose 1,6-bisphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyoglomus thermophilum | - |
- |
- |
Dictyoglomus thermophilum Rt46 B.1 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
not sensitive to O2 | Dictyoglomus thermophilum |
Purification (Comment) | Organism |
---|---|
231fold, to homogeneity | Dictyoglomus thermophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.4 | - |
purified enzyme | Dictyoglomus thermophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(polyphosphate)n + D-fructose 6-phosphate | n: 12-18 | Dictyoglomus thermophilum | (polyphosphate)n-1 + D-fructose 1,6-bisphosphate | - |
r | |
(polyphosphate)n + D-fructose 6-phosphate | 75% of activity with diphosphate | Dictyoglomus thermophilum | (polyphosphate)n-1 + D-fructose 1,6-bisphosphate | - |
r | |
(polyphosphate)n + D-fructose 6-phosphate | n: 12-18 | Dictyoglomus thermophilum Rt46 B.1 | (polyphosphate)n-1 + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-fructose 6-phosphate | - |
Dictyoglomus thermophilum | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-fructose 6-phosphate | equilibrium lies in forward direction | Dictyoglomus thermophilum | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-fructose 6-phosphate | specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate | Dictyoglomus thermophilum | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-fructose 6-phosphate | - |
Dictyoglomus thermophilum Rt46 B.1 | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-fructose 6-phosphate | equilibrium lies in forward direction | Dictyoglomus thermophilum Rt46 B.1 | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-fructose 6-phosphate | specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate | Dictyoglomus thermophilum Rt46 B.1 | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
triphosphate + D-fructose 6-phosphate | 82% of activity with diphosphate | Dictyoglomus thermophilum | diphosphate + D-fructose 1,6-bisphosphate | - |
r | |
triphosphate + D-fructose 6-phosphate | 82% of activity with diphosphate | Dictyoglomus thermophilum Rt46 B.1 | diphosphate + D-fructose 1,6-bisphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Dictyoglomus thermophilum |
Synonyms | Comment | Organism |
---|---|---|
PPi-dependent phosphofructokinase | - |
Dictyoglomus thermophilum |
PPi-PFK | - |
Dictyoglomus thermophilum |
pyrophosphate-dependent phosphofructokinase | - |
Dictyoglomus thermophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Dictyoglomus thermophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: 150 min | Dictyoglomus thermophilum |
90 | - |
half-life: 10 min | Dictyoglomus thermophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | 6.3 | 50°C, forward reaction | Dictyoglomus thermophilum |
7 | 7.5 | 50°C, reverse reaction | Dictyoglomus thermophilum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Dictyoglomus thermophilum | - |
- |
4.4 |