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Literature summary for 2.7.1.90 extracted from

  • Roberson, R.S.; Ronimus, R.S.; Gephard, S.; Morgan, H.W.
    Biochemical characterization of an active pyrophosphate-dependent phosphofructokinase from Treponema pallidum (2001), FEMS Microbiol. Lett., 194, 257-260.
    View publication on PubMed
Search for more literature for 2.7.1.90

Cloned(Commentary)

Cloned (Comment) Organism
gene TP0542, DNA sequence determination, expression in Escherichia coli DH5alpha Treponema pallidum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ complete inhibition at 1 mM Treponema pallidum
Cu2+ 85% inhibition at 0.2 mM Treponema pallidum
Mn2+
-
 Treponema pallidum
Zn2+
-
 Treponema pallidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 diphosphate pH 7.0, 37°C Treponema pallidum
0.27
-
 D-fructose1,6-bisphosphate pH 8.0, 37°C Treponema pallidum
0.53
-
 D-fructose 6-phosphate pH 7.0, 37°C Treponema pallidum
1.39
-
 phosphate pH 8.0, 37°C Treponema pallidum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Treponema pallidum

Organism

Organism UniProt Comment Textmining
Treponema pallidum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Treponema pallidum phosphate + D-fructose 1,6-bisphosphate
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Treponema pallidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 forward reaction Treponema pallidum
7.8 8.3 reverse reaction Treponema pallidum