Literature summary for 2.7.1.90 extracted from

Saavedra, E.; Encalada, R.; Pineda, E.; Jasso-Chavez, R.; Moreno-Sanchez, R.
Glycolysis in Entamoeba histolytica. Biochemical characterization of recombinant glycolytic enzymes and flux control analysis (2005), FEBS J., 272, 1767-1783.

Search for more literature for 2.7.1.90

Cloned(Commentary)

Cloned (Comment)	Organism
-	Entamoeba histolytica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	diphosphate	37°C, pH 7.0	Entamoeba histolytica
0.38	-	diphosphate	37°C, pH 6.0	Entamoeba histolytica
0.455	-	D-fructose 6-phosphate	37°C, pH 7.0	Entamoeba histolytica
0.695	-	D-fructose 6-phosphate	37°C, pH 6.0	Entamoeba histolytica

Organism

Organism	UniProt	Comment	Textmining
Entamoeba histolytica	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Entamoeba histolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
112	-	37°C, pH 6.0	Entamoeba histolytica
298	-	37°C, pH 7.0	Entamoeba histolytica

Storage Stability

Storage Stability	Organism
-20°C, 50% v/v glycerol, less than 50% loss of activity within 2 months	Entamoeba histolytica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diphosphate + D-fructose 6-phosphate	-	Entamoeba histolytica	phosphate + D-fructose 1,6-diphosphate	-	r
phosphate + D-fructose 1,6-diphosphate	-	Entamoeba histolytica	diphosphate + D-fructose 6-phosphate	-	r

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8	synthesis of diphosphate	Entamoeba histolytica
6.8	7.4	synthesis of D-fructose 1,6-diphosphate	Entamoeba histolytica

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Release 2023.1 (January 2023)