Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Methylococcus capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of citrate, pyruvate, phosphoenolpyruvate, L-serine, phosphoglycerate, ATP, ADP, AMP, NADP, NADs and NADPH to the assay mixture has no effect on activity | Methylococcus capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
sedoheptulose 7-phosphate | in 0.1 M HEPES-HCl, pH 7.0, at 30°C | Methylococcus capsulatus | |
0.027 | - |
diphosphate | using D-fructose 6-phosphate as cosubstrate, in 0.1 M HEPES-HCl, pH 7.0, at 30°C | Methylococcus capsulatus | |
0.328 | - |
D-fructose 1,6-bisphosphate | in 0.1 M HEPES-HCl, pH 7.0, at 30°C | Methylococcus capsulatus | |
2.27 | - |
D-fructose 6-phosphate | in 0.1 M HEPES-HCl, pH 7.0, at 30°C | Methylococcus capsulatus | |
8.69 | - |
phosphate | using D-fructose 1,6-bisphosphate as cosubstrate, in 0.1 M HEPES-HCl, pH 7.0, at 30°C | Methylococcus capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for maximal activity, Co2+ or Mn2+ can substitute for Mg2+ with 63% and 48% of maximal activity | Methylococcus capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Methylococcus capsulatus |
90000 | - |
gel filtration | Methylococcus capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | strong specificity for diphosphate as a phosphoryl donor | Methylococcus capsulatus | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-ribulose 5-phosphate | - |
Methylococcus capsulatus | phosphate + ? | - |
? | |
diphosphate + sedoheptulose 7-phosphate | - |
Methylococcus capsulatus | phosphate + ? | - |
? | |
additional information | ATP, ADP, AMP, CTP, CDP, CMP, UTP, UDP, UMP, GTP, GDP, and GMP at 1 mM as well as polyphosphates (n=3, n=9, n=15 and n=45) at 0.1 mg/ml cannot substitute for diphosphate, D-glucose 6-phosphate, D-glucose 1-phosphate, D-fructose 1-phosphate, D-mannose 6-phosphate, D-ribose 5-phosphate, D-ribose 1-phosphate, or D-erythrose 4-phosphate cannot substitute for D-fructose 6-phosphate as a phosphate acceptor | Methylococcus capsulatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, SDS-PAGE | Methylococcus capsulatus |
Synonyms | Comment | Organism |
---|---|---|
PPi-PFK | - |
Methylococcus capsulatus |
pyrophosphate-dependent 6-phosphofructokinase | - |
Methylococcus capsulatus |
pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase | - |
Methylococcus capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Methylococcus capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Methylococcus capsulatus |