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Literature summary for 2.7.1.90 extracted from
- Characterization of recombinant PPi-dependent 6-phosphofructokinases from Methylosinus trichosporium OB3b and Methylobacterium nodulans ORS 2060 (2012), Biochemistry (Moscow), 77, 288-295.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Methylosinus trichosporium |
| expressed in Escherichia coli BL21(DE3) cells | Methylobacterium nodulans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 50% inhibition in the forward reaction and complete inhibition in the reverse reaction at 1 mM | Methylosinus trichosporium |  |
| AMP | 90% inhibition in the forward reaction and complete inhibition in the reverse reaction at 1 mM | Methylosinus trichosporium | |
| additional information | not inhibited by ADP or AMP | Methylobacterium nodulans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
diphosphate | at pH 7.5 and 30°C | Methylobacterium nodulans | |
| 0.011 | - |
diphosphate | at pH 7.5 and 30°C | Methylosinus trichosporium | |
| 0.043 | - |
D-fructose 1,6-bisphosphate | at pH 7.5 and 30°C | Methylobacterium nodulans | |
| 0.07 | - |
D-fructose 1,6-bisphosphate | at pH 7.5 and 30°C | Methylosinus trichosporium | |
| 0.21 | - |
D-sedoheptulose 7-phosphate | at pH 7.5 and 30°C | Methylobacterium nodulans | |
| 0.24 | - |
D-fructose 6-phosphate | at pH 7.5 and 30°C | Methylosinus trichosporium | |
| 0.51 | - |
D-sedoheptulose 7-phosphate | at pH 7.5 and 30°C | Methylosinus trichosporium | |
| 0.65 | - |
D-fructose 6-phosphate | at pH 7.5 and 30°C | Methylobacterium nodulans | |
| 1.04 | - |
phosphate | at pH 7.5 and 30°C | Methylobacterium nodulans | |
| 2.4 | - |
phosphate | at pH 7.5 and 30°C | Methylosinus trichosporium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | at 1 mM concentration Co2+ can replace Mg2+ (about 50% of Mg2+ activity) | Methylobacterium nodulans | |
| Mg2+ | required, Km values of 0.03 mM (forward reaction) and 0.363 mM (reverse reaction) at pH 7.5 and 50°C | Methylobacterium nodulans | |
| Mg2+ | required, Km values of 0.047 mM (forward reaction) and 0.36 mM (reverse reaction) at pH 7.5 and 30°C | Methylosinus trichosporium | |
| Mn2+ | at 1 mM concentration Mn2+ can replace Mg2+ (81% of Mg2+ activity) | Methylosinus trichosporium | |
| Mn2+ | at 1 mM concentration Mn2+ can replace Mg2+ (about 65% of Mg2+ activity) | Methylobacterium nodulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
4 * 43000, SDS-PAGE | Methylobacterium nodulans |
| 45000 | - |
6 * 45000, SDS-PAGE | Methylosinus trichosporium |
| 180000 | - |
gel filtration | Methylobacterium nodulans |
| 270000 | - |
gel filtration | Methylosinus trichosporium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylobacterium nodulans | B8IF02 | - |
- |
| Methylobacterium nodulans ORS 2060 | B8IF02 | - |
- |
| Methylosinus trichosporium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+NTA column chromatography, gel filtration | Methylosinus trichosporium |
| Ni2+NTA column chromatography, gel filtration | Methylobacterium nodulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | the enzyme is highly specific to diphosphate as the phosphoryl donor | Methylosinus trichosporium | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | the enzyme is highly specific to diphosphate as the phosphoryl donor | Methylobacterium nodulans | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | the enzyme is highly specific to diphosphate as the phosphoryl donor | Methylobacterium nodulans ORS 2060 | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-ribulose 5-phosphate | 1.4% activity compared to D-fructose 6-phosphate | Methylosinus trichosporium | ? | - |
? | |
| diphosphate + D-sedoheptulose 7-phosphate | - |
Methylosinus trichosporium | phosphate + D-sedoheptulose 1,7-bisphosphate | - |
? | |
| diphosphate + D-sedoheptulose 7-phosphate | - |
Methylobacterium nodulans | phosphate + D-sedoheptulose 1,7-bisphosphate | - |
? | |
| diphosphate + D-sedoheptulose 7-phosphate | - |
Methylobacterium nodulans ORS 2060 | phosphate + D-sedoheptulose 1,7-bisphosphate | - |
? | |
| additional information | D-glucose 6-phosphate, D-glucose1-phosphate, D-fructose1-phosphate, D-mannose6-phosphate, ribose5-phosphate, ribose1-phosphate, and D-erythrose4-phosphate are not effective as the phosphoryl acceptors for the enzyme | Methylosinus trichosporium | ? | - |
? | |
| additional information | D-glucose 6-phosphate, D-glucose1-phosphate, D-fructose1-phosphate, D-mannose6-phosphate, ribose5-phosphate, ribose1-phosphate, and D-erythrose4-phosphate are not effective as the phosphoryl acceptors for the enzyme. No activity is observed with ribulose 5-phosphate | Methylobacterium nodulans | ? | - |
? | |
| additional information | D-glucose 6-phosphate, D-glucose1-phosphate, D-fructose1-phosphate, D-mannose6-phosphate, ribose5-phosphate, ribose1-phosphate, and D-erythrose4-phosphate are not effective as the phosphoryl acceptors for the enzyme. No activity is observed with ribulose 5-phosphate | Methylobacterium nodulans ORS 2060 | ? | - |
? | |
| phosphate + D-fructose 1,6-bisphosphate | - |
Methylosinus trichosporium | diphosphate + D-fructose 6-phosphate | - |
r | |
| phosphate + D-fructose 1,6-bisphosphate | - |
Methylobacterium nodulans | diphosphate + D-fructose 6-phosphate | - |
r | |
| phosphate + D-fructose 1,6-bisphosphate | - |
Methylobacterium nodulans ORS 2060 | diphosphate + D-fructose 6-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | 6 * 45000, SDS-PAGE | Methylosinus trichosporium |
| homotetramer | 4 * 43000, SDS-PAGE | Methylobacterium nodulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPi-dependent 6-phosphofructokinase | - |
Methylosinus trichosporium |
| PPi-dependent 6-phosphofructokinase | - |
Methylobacterium nodulans |
| PPi-PFK | - |
Methylosinus trichosporium |
| PPi-PFK | - |
Methylobacterium nodulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Methylobacterium nodulans |
| 50 | - |
- |
Methylosinus trichosporium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Methylosinus trichosporium |
| 7.5 | - |
- |
Methylobacterium nodulans |
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