Cloned (Comment) | Organism |
---|---|
expression of wild-type FLT-3 and mutant FLT-3 ITD in HEK293 cells, expression of hemagglutinin-tagged wild-type FLT-3, containing an ER-targeting sequence, in THP-1 cells, expression of hemagglutinin-tagged or EGFP-tagged, connected via a six-glycine-linker, FLT-3 in COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K634A | site-directed mutagenesis PDGFbeta receptor tyrosine kinase, results in impaired tyrosine autophosphorylation and maturation, as well as loss of kinase activity | Homo sapiens |
K644A | site-directed mutagenesis of juxtamembrane tandem mutagenic construct of FLT-3 RTK and of wild-type FLT-3 RTK, maturation of the mutants by glycosylation and phosphorylation is impaired, altered localization compaired to the wild-type enzyme, overview | Homo sapiens |
additional information | construction of FLT-3 with a tandem repeat of the juxtamembrane, termed FLT-3 ITD RTK, showing impaired maturation during recombinant expression in MV4-11 cells, the mutation alters enzyme interactions with other proteins, e.g. heat shock proteins, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AG1296 | FLT-3 inhibitor treatment results in formation of larger amounts of the mature 150 kDa form of mutant FLT-3 ITD | Homo sapiens | |
SU11248 | FLT-3 inhibitor treatment results in formation of larger amounts of the mature 150 kDa form of mutant FLT-3 ITD | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
x * 130000, recombinant FLT-3 ITD mutant, SDS-PAGE, x * 150000, recombinant wild-type FLT-3, SDS-PAGE | Homo sapiens |
150000 | - |
x * 130000, recombinant FLT-3 ITD mutant, SDS-PAGE, x * 150000, recombinant wild-type FLT-3, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a [protein]-L-tyrosine | Homo sapiens | FLT-3 is involved in signal transduction, mechanisms | ADP + a [protein]-L-tyrosine phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | FLT-3 and FLT-3 ITD are high-mannose-type glycoproteins | Homo sapiens |
phosphoprotein | tyrosine autophosphorylation regulates maturation of the FLT-3 RTK and of PDGFbeta receptor | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a [protein]-L-tyrosine | - |
Homo sapiens | ADP + a [protein]-L-tyrosine phosphate | - |
? | |
ATP + a [protein]-L-tyrosine | FLT-3 is involved in signal transduction, mechanisms | Homo sapiens | ADP + a [protein]-L-tyrosine phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 130000, recombinant FLT-3 ITD mutant, SDS-PAGE, x * 150000, recombinant wild-type FLT-3, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
FLT-3 | - |
Homo sapiens |
fms-like tyrosine kinase 3 | - |
Homo sapiens |
receptor tyrosine kinase | - |
Homo sapiens |
RTK | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |