Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | hypoxia/reoxygenation induces tyrosine phosphorylation of Lck and Lck activity in breast cancer cells and of IkappaBalpha, but not of IkappaBalpha serine phosphorylation, activation is inhibited by emodin and pp2 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type Lck in Y394F Lck mutant cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y394F | Lck F394 mutant cells show reduced tyrosine phosphorylation of the enzyme, expression of recombinant wild-type Lck in the mutant cells increases the autophosphorylation activity 3fold | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-amino-5-(4-chlorophenyl)-7-(tert-butyl)pyrazolo[3,4-d]pyrimidine | i.e. pp2, Lck inhibitor | Homo sapiens | |
emodin | i.e. 6-methyl-1,3,8-trihydroxyanthraquinone, Lck inhibitor | Homo sapiens | |
additional information | regulation of Lck by phosphorylation at Tyr505 by C-terminal Scr protein-tyrosine kinase which inhibits the enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mn2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + IkappaBalpha-L-tyrosine | Homo sapiens | p56lck regulates cell motility and nuclear factor kappaB-mediated secretion of urokinase type plasminogen activator through tyrosine phosphorylation of IkappaBalpha following hypoxia/reoxygenation, molecular mechanism, physiological role | ADP + IkappaBalpha-L-tyrosine phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | regulation of Lck by phosphorylation at Tyr505 by C-terminal Scr protein-tyrosine kinase which inhibits the enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast cancer cell | - |
Homo sapiens | - |
MCF-7 cell | low invasive breast cancer cell | Homo sapiens | - |
MDA-MB-231 cell | high invasive breast cancer cell | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + IkappaBalpha-L-tyrosine | p56lck regulates cell motility and nuclear factor kappaB-mediated secretion of urokinase type plasminogen activator through tyrosine phosphorylation of IkappaBalpha following hypoxia/reoxygenation, molecular mechanism, physiological role | Homo sapiens | ADP + IkappaBalpha-L-tyrosine phosphate | - |
? | |
ATP + IkappaBalpha-L-tyrosine | Lck interacts via its SH2 domain with tyrosine-phosphorylated IkappaBalpha | Homo sapiens | ADP + IkappaBalpha-L-tyrosine phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Lck | - |
Homo sapiens |
More | Lck is a member of the Src non-receptor tyrosine kinase family | Homo sapiens |
p56lck | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |