Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.10.2 extracted from

  • Meyer, A.N.; Gastwirt, R.F.; Schlaepfer, D.D.; Donoghue, D.J.
    The cytoplasmic tyrosine kinase Pyk2 as a novel effector of fibroblast growth factor receptor 3 activation (2004), J. Biol. Chem., 279, 28450-28457.
    View publication on PubMed
Search for more literature for 2.7.10.2

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of Myc-tagged wild-type non-receptor tyrosine kinase Pyk2 and mutant enzyme forms in 293T cells, wild-type enzyme expression leads to increased activation of Stat5B, cotransfection with wild-type FGFR1-4, FGFR3 mutant K650E, a myristoylated FGFR, and truncated FGFR3 in 293T cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K457A site-directed mutagenesis, mutation leads to increase in phosphorylation sites on the substrate FGFR3 Homo sapiens
additional information construction of a Pyk2 mutant Pyk2-DELTAKIN lacking the kinase domain resulting in loss of interaction and phosphorylation activity with FGFR3 Homo sapiens
Y402F site-directed mutagenesis, mutation leads to increase in phosphorylation sites on the substrate FGFR3 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [fibroblast growth factor receptor FGFR2]-L-tyrosine Homo sapiens activation by non-receptor tyrosine kinase Pyk2, interaction via the FGFR juxtamembrane and the Pyk2 kinase domain, activation is antagonized by tyrosine phosphatase Shp2 ADP + [fibroblast growth factor receptor FGFR2]-L-tyrosine phosphate
-
 ?
ATP + [fibroblast growth factor receptor FGFR3]-L-tyrosine Homo sapiens activation by non-receptor tyrosine kinase Pyk2, interaction via the FGFR juxtamembrane and the Pyk2 kinase domain, activation is antagonized by tyrosine phosphatase Shp2 ADP + [fibroblast growth factor receptor FGFR3]-L-tyrosine phosphate
-
 ?
ATP + [Stat5B]-L-tyrosine Homo sapiens activation by non-receptor tyrosine kinase Pyk2 ADP + [Stat5B]-L-tyrosine phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein Pyk2 performs essential autophosphorylation at Tyr402, this phosphorylation step is not essential in case of FRGFR3 activation by phosphorylation through Pyk2, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [fibroblast growth factor receptor FGFR2]-L-tyrosine activation by non-receptor tyrosine kinase Pyk2, interaction via the FGFR juxtamembrane and the Pyk2 kinase domain, activation is antagonized by tyrosine phosphatase Shp2 Homo sapiens ADP + [fibroblast growth factor receptor FGFR2]-L-tyrosine phosphate
-
 ?
ATP + [fibroblast growth factor receptor FGFR2]-L-tyrosine activation by non-receptor tyrosine kinase Pyk2, interaction via the FGFR juxtamembrane and the Pyk2 kinase domain Homo sapiens ADP + [fibroblast growth factor receptor FGFR2]-L-tyrosine phosphate
-
 ?
ATP + [fibroblast growth factor receptor FGFR3]-L-tyrosine activation by non-receptor tyrosine kinase Pyk2, interaction via the FGFR juxtamembrane and the Pyk2 kinase domain, activation is antagonized by tyrosine phosphatase Shp2 Homo sapiens ADP + [fibroblast growth factor receptor FGFR3]-L-tyrosine phosphate
-
 ?
ATP + [fibroblast growth factor receptor FGFR3]-L-tyrosine activation by non-receptor tyrosine kinase Pyk2, interaction via the FGFR juxtamembrane and the Pyk2 kinase domain Homo sapiens ADP + [fibroblast growth factor receptor FGFR3]-L-tyrosine phosphate
-
 ?
ATP + [Stat5B]-L-tyrosine activation by non-receptor tyrosine kinase Pyk2 Homo sapiens ADP + [Stat5B]-L-tyrosine phosphate
-
 ?
additional information Pyk2 performs autophosphorylation at Tyr402, fibroblast growth factor receptors FGFR1 and FGFR4 are no substrates of Pyk2 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
non-receptor protein-tyrosine kinase
-
 Homo sapiens
proline-rich tyrosin ekinase 2
-
 Homo sapiens
Pyk2
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens