Application | Comment | Organism |
---|---|---|
synthesis | expression and a two-step purification procedure for the doubly tagged full-length expression construct, H6-FL-TYK-2-FLAG. In the presence of ATP and a peptide substrate, H6-FL-TYK-2-FLAG shows a marked lag in phosphopeptide product formation, while the wild-type shows no such lag. The lag can be eliminated by ATP pretreatment. The potencies of several nanomolar inhibitors are similar for TYK-2 KD and H6-FL-TYK-2-FLAG. However, these same inhibitors are about 1000 times less potent inhibiting the autophosphorylation of H6-FL-TYK-2-FLAG than they are inhibiting the phosphorylation of a peptide substrate modeled after the activation loop sequence of wild-type TYK-2 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression and a two-step purification procedure for the doubly tagged full-length expression construct, H6-FL-TYK-2-FLAG. In the presence of ATP and a peptide substrate, H6-FL-TYK-2-FLAG shows a marked lag in phosphopeptide product formation, while the wild-type shows no such lag. The lag can be eliminated by ATP pretreatment. The potencies of several nanomolar inhibitors are similar for TYK-2 KD and H6-FL-TYK-2-FLAG. However, these same inhibitors are about 1000 times less potent inhibiting the autophosphorylation of H6-FL-TYK-2-FLAG than they are inhibiting the phosphorylation of a peptide substrate modeled after the activation loop sequence of wild-type TYK-2 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P29597 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
TYK-2 | - |
Homo sapiens |
tyrosine kinase 2 | - |
Homo sapiens |