Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | autophosphorylation patterns for activation of PknB, PknD, PknE, and PknF, e.g. PknB phosphorylation at Thr171 and Thr173 of the activation loop and at Thr294 of the juxtamembrane domain are necessary for activity | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged PknB, PknD, PknE, and PknF in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mycobacterium tuberculosis | 16020 | - |
additional information | PknB, PknD, PknE, and PknF possess an N-terminal kinase domain, a juxtamembrane domain of varying length, and an activation loop | Mycobacterium tuberculosis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | PknB, PknD, PknE, and PknF are autophosphorylated at multiple sites, phosphorylation patterns, overview, the enzymes are dephosphorylated by the cognate mycobacterial phosphatase PstP | Mycobacterium tuberculosis |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged PknB, PknD, PknE, and PknF rom Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myelin basic protein | PknB, PknD, PknE, and PknF | Mycobacterium tuberculosis | ADP + phosphorylated myelin basic protein | - |
? | |
additional information | PknB, PknD, PknE, and PknF perform autophosphorylation | Mycobacterium tuberculosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | PknB, PknD, PknE, and PknF possess an N-terminal kinase domain, a juxtamembrane domain of varying length, and an activation loop | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
PknB | - |
Mycobacterium tuberculosis |
PknD | - |
Mycobacterium tuberculosis |
PknE | - |
Mycobacterium tuberculosis |
PknF | - |
Mycobacterium tuberculosis |
Ser/Thr protein kinase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |