Cloned (Comment) | Organism |
---|---|
expression of WNK1 and truncated WNK4 comprising residues 1-593 in HEK-293 cells, co-expression of truncated WNK1 comprising residues 1-661 with claudin-4, oxidative stress response kinase 1 OSR1, STE/SPS1-related proline/alanine-rich kinase SPAK, and the Na+-K+-2Cl- co-transporter-1-(1-260) construct NKCC1-(1-260) in Escherichia coli strain BL21 as GST-fusion proteins | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
WNK1 partially from testis, recombinant GST-fusion truncated WNK1 comprising residues 1-661 with claudin-4, wild-type and mutant oxidative stress response kinase 1 OSR1, STE/SPS1-related proline/alanine-rich kinase SPAK, and Na+-K+-2Cl- co-transporter-1-(1-260) construct NKCC1-(1-260) from Escherichia coli by glutathione affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose tissue | very low expression level of WNK1 | Rattus norvegicus | - |
brain | very low expression level of WNK1 | Rattus norvegicus | - |
heart | very low expression level of WNK1 | Rattus norvegicus | - |
liver | very low expression level of WNK1 | Rattus norvegicus | - |
pancreas | low expression level of WNK1 | Rattus norvegicus | - |
spleen | very low expression level of WNK1 | Rattus norvegicus | - |
testis | WNK4, high expression level of WNK1 | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + claudin-4 | recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli | Rattus norvegicus | ADP + phosphorylated claudin-4 | - |
? | |
ATP + myelin basic protein | recombinant WNK1 or WNK4 expressed in Escherichia coli | Rattus norvegicus | ADP + phosphorylated myelin basic protein | - |
? | |
ATP + NKCC1-(1-260) | i.e. Na+-K+-2Cl- co-transporter-1-(1-260) construct, recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli | Rattus norvegicus | ADP + phosphorylated NKCC1-(1-260) | - |
? | |
ATP + oxidative stress response kinase 1 OSR1 | recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli, phosphorylation at Ser325 and Thr185, the latter is required for activation of OSR1 | Rattus norvegicus | ADP + phosphorylated oxidative stress response kinase 1 OSR1 | - |
? | |
ATP + STE/SPS1-related proline/alanine-rich kinase SPAK | recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli | Rattus norvegicus | ADP + phosphorylated STE/SPS1-related proline/alanine-rich kinase SPAK | - |
? | |
additional information | WNK1 interacts with OSR1 and SPAK, overview | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
WNK1 | - |
Rattus norvegicus |
WNK4 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |