Cloned (Comment) | Organism |
---|---|
gene Rv1266c or pknH, overexpression of wild-type and mutant GST-fusion PknH in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
K45M | site-directed mutagenesis, inactive mutant | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H-7 | - |
Mycobacterium tuberculosis | |
additional information | enzyme expression level is decreased under low pH and heat shock conditions | Mycobacterium tuberculosis | |
staurosporine | - |
Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | PknH is a transmembrane protein | Mycobacterium tuberculosis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ or Mn2+, dependent on | Mycobacterium tuberculosis | |
Mn2+ | Mg2+ or Mn2+, dependent on | Mycobacterium tuberculosis | |
additional information | no activity with cations other than Mg2+ or Mn2+ | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
x * 97000, recombinant GST-fusion PknH, SDS-PAGE, x * 68000, non-tagged PknH, SDS-PAGE | Mycobacterium tuberculosis |
97000 | - |
x * 97000, recombinant GST-fusion PknH, SDS-PAGE, x * 68000, non-tagged PknH, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycobacterium tuberculosis | enzyme is involved in stress response and adaptation to environmental changes in mycobacteria | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
strains H37Rv and H37Ra, PknH is specific for mycobacteria of the Mycobacterium tuberculosis complex | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | PknH performs autophosphorylation, mutant K45M is inactive | Mycobacterium tuberculosis |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant GST-fusion PknH from Escherichia coli by glutathione affinity chromatography | Mycobacterium tuberculosis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate | Lys45 is important for PknH catalytic and autophosphorylation activity | Mycobacterium tuberculosis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme expression level under stress conditions, overview | Mycobacterium tuberculosis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + histone | - |
Mycobacterium tuberculosis | ADP + phosphorylated histone | - |
? | |
ATP + myelin basic protein | - |
Mycobacterium tuberculosis | ADP + phosphorylated myelin basic protein | - |
? | |
additional information | enzyme is involved in stress response and adaptation to environmental changes in mycobacteria | Mycobacterium tuberculosis | ? | - |
? | |
additional information | PknH performs autophosphorylation | Mycobacterium tuberculosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 97000, recombinant GST-fusion PknH, SDS-PAGE, x * 68000, non-tagged PknH, SDS-PAGE | Mycobacterium tuberculosis |
More | PknH contains 11 subdomains, schematic structure, overview | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
Hank's type serine/threonine kinase | - |
Mycobacterium tuberculosis |
PknH | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycobacterium tuberculosis | amino acid sequence calculation | - |
6.3 |