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Literature summary for 2.7.11.1 extracted from
- A phosphoprotein from the archaeon Sulfolobus solfataricus with protein-serine/threonine kinase activity (2004), J. Bacteriol., 186, 463-472.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ORF sso0469, amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli | Saccharolobus solfataricus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D349A | site-directed mutagenesis, inactive mutant | Saccharolobus solfataricus |
| D394A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-tpe enzyme | Saccharolobus solfataricus |
| K215A | site-directed mutagenesis, inactive mutant | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tamoxifen | - |
Saccharolobus solfataricus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | associated | Saccharolobus solfataricus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | less effective than Mn2+ | Saccharolobus solfataricus | |
| Mn2+ | preferred metal ion cofactor | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
x * 67000, glycosylated and phosphorylated PK3, SDS-PAGE | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | Saccharolobus solfataricus | - |
ADP + a phosphoprotein | - |
? | |
| ATP + a protein | Saccharolobus solfataricus P2 | - |
ADP + a phosphoprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
ORF sso0469 encodes PK3 | - |
| Saccharolobus solfataricus P2 | - |
ORF sso0469 encodes PK3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme partially by detergent solublization from membranes and anion exchange chromatography | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | - |
Saccharolobus solfataricus | ADP + a phosphoprotein | - |
? | |
| ATP + a protein | phosphorylation of exogenous substrates takes place on serine and, occasionally, threonine, but the enzyme displays a strong preference for threonine as the phospho-acceptor amino acid residue, overview, no activity with GTP except for the autophosphorylation reaction in vitro which functions with GTP and also GDP | Saccharolobus solfataricus | ADP + a phosphoprotein | - |
? | |
| ATP + a protein | - |
Saccharolobus solfataricus P2 | ADP + a phosphoprotein | - |
? | |
| ATP + a protein | phosphorylation of exogenous substrates takes place on serine and, occasionally, threonine, but the enzyme displays a strong preference for threonine as the phospho-acceptor amino acid residue, overview, no activity with GTP except for the autophosphorylation reaction in vitro which functions with GTP and also GDP | Saccharolobus solfataricus P2 | ADP + a phosphoprotein | - |
? | |
| ATP + bovine serum albumin | - |
Saccharolobus solfataricus | ADP + phosphorylated bovine serum albumin | - |
? | |
| ATP + bovine serum albumin | - |
Saccharolobus solfataricus P2 | ADP + phosphorylated bovine serum albumin | - |
? | |
| ATP + casein | - |
Saccharolobus solfataricus | ADP + phosphocasein | - |
? | |
| ATP + casein | - |
Saccharolobus solfataricus P2 | ADP + phosphocasein | - |
? | |
| ATP + myelin basic protein | - |
Saccharolobus solfataricus | ADP + phosphorylated myelin basic protein | - |
? | |
| ATP + myelin basic protein | - |
Saccharolobus solfataricus P2 | ADP + phosphorylated myelin basic protein | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 67000, glycosylated and phosphorylated PK3, SDS-PAGE | Saccharolobus solfataricus |
| More | enzyme domain structure analysis | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| membrane-associated protein-serine/threonine kinase | - |
Saccharolobus solfataricus |
| SsoPK3 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Saccharolobus solfataricus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharolobus solfataricus | |
| additional information | no activity with GTP | Saccharolobus solfataricus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharolobus solfataricus | PK3, isoelectric focusing | - |
5.7 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
pH 6.5, 35°C, recombinant enzyme | Saccharolobus solfataricus | tamoxifen | |
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