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Literature summary for 2.7.11.1 extracted from
- Characterization of Arabidopsis calcium-dependent protein kinases: activated or not by calcium? (2012), Biochem. J., 447, 291-299.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozyme quantitative real-time PCR expression analysis, overexpression of 14 isozymes as HA-tagged proteins, driven by the 35S promoter, in transgenic Arabidopsis thaliana plants using Agrobacterium tumefaciens strain GV3101 for transformation, most of them are partially or mainly associated with membranes, in agreement with acylation predictions | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | all isozymes, except for CPK11 and CPK4 | Arabidopsis thaliana | 16020 | - |
| additional information | subcellular localizationof isozymes, overview | Arabidopsis thaliana | - |
- |
| soluble | isozymes CPK2, 3, 4, 5, 11, and 19 | Arabidopsis thaliana | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | dependent on, the enzymes contain calmodulin-like calcium binding domains, the EF-hands. The plant isozymes display highly variable calcium-dependences for their kinase activities: seven CPKs from subgroups 1 and 2 are clearly sensitive to calcium with different intensities, whereas six CPKs from subgroup 3 exhibit low or no calcium sensitivity to two generic substrates. But all plant isozymes bind calcium, except for CPK25, which is calcium-independent owing to the absence of functional EF-hands. Calcium binding differentially affects CDPK isoforms that may be activated by distinct molecular mechanisms | Arabidopsis thaliana |  |
| Mg2+ | required | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
14 isozymes of the three main CDPK subgroups: CPKs 2, 4, 5, 11 and 25 from subgroup 1, CPKs 3, 9 and 19 from subgroup 2, and CPKs 7, 8, 10, 13, 30 and 32 from subgroup 3 | - |
| Arabidopsis thaliana Col0 | - |
14 isozymes of the three main CDPK subgroups: CPKs 2, 4, 5, 11 and 25 from subgroup 1, CPKs 3, 9 and 19 from subgroup 2, and CPKs 7, 8, 10, 13, 30 and 32 from subgroup 3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant HA-tagged isozymes from transgenic overexpressing Arabidopsis thaliana plants by gel filtration and immunoaffinity chromatography on an anti-HA resin | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + histone | - |
Arabidopsis thaliana | ADP + phosphorylated histone | - |
? | |
| ATP + histone | - |
Arabidopsis thaliana Col0 | ADP + phosphorylated histone | - |
? | |
| ATP + syntide-2 | very low activity with iozyme CPK13, higher activity with isozyme CPK19, isozyme specific activities, overview | Arabidopsis thaliana | ADP + phosphorylated syntide-2 | - |
? | |
| ATP + syntide-2 | very low activity with iozyme CPK13, higher activity with isozyme CPK19, isozyme specific activities, overview | Arabidopsis thaliana Col0 | ADP + phosphorylated syntide-2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | CDPKs consist of a variable N-terminal domain, a conserved serine/threonine kinase domain followed by an autoinhibitory junction region and a C-terminal regulatory CaMLD (CaM-like domain) | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| calcium-dependent protein kinase | - |
Arabidopsis thaliana |
| CDPK | - |
Arabidopsis thaliana |
| CPK | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | calcium-dependent protein kinases, which contain both calmodulin-like calcium binding and serine/threonine protein kinase domains, are only present in plants and some protozoans | Arabidopsis thaliana |
| additional information | calcium binding differentially affects CDPK isoforms that may be activated by distinct molecular mechanisms | Arabidopsis thaliana |
| physiological function | upon activation by a stimulus, calcium-dependent protein kinases transduce the signal through phosphorylation cascades to induce downstream responses, including transcriptional regulation | Arabidopsis thaliana |
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